Assessment of tumoral PD-L1 expression and intratumoral CD8+ T cells in urothelial carcinoma.
Assessment of tumoral PD-L1 expression and intratumoral CD8+ T cells in urothelial carcinoma.
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DOI：
10.1016/j.urology.2014.10.020
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发表时间：
2015-03
期刊：
影响因子：
2.1
通讯作者：
Netto GJ
中科院分区：
文献类型：
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作者：
Faraj SF;Munari E;Guner G;Taube J;Anders R;Hicks J;Meeker A;Schoenberg M;Bivalacqua T;Drake C;Netto GJ
评估膀胱尿路上皮癌中程序性死亡配体 1 (PD-L1) 的表达与肿瘤浸润 CD8+ T 细胞的关系。组织微阵列是由我们医院（1994-2002）进行的 56 例膀胱切除标本制备的。使用鼠抗人 PD-L1 单克隆抗体 5H1 评估 PD-L1 免疫表达。指定每个点的膜 PD-L1 表达范围。显示≥5%表达的斑点被认为是阳性的。还计算了每个肿瘤的平均 PD-L1 表达（5% 阳性截止值）。 “高 CD8 密度”定义为给定点中每个高倍视野中存在 ≥60 个 CD8+ 上皮内淋巴细胞。如果 ≥50% 的斑点为高密度，则肿瘤被认为是高密度。大约 20% 的肿瘤中 PD-L1 表达呈阳性。良性尿路上皮斑点均不表达 PD-L1。在大约 20% 的病例中观察到高 CD8 密度。 CD8 密度与 PD-L1 表达不相关。总生存率 (OS) 和疾病特异性生存率 (DSS) 分别为 14% 和 28%（中位随访时间为 31.5 个月）。 PD-L1 表达与膀胱切除术年龄相关 (P = .01)。其余临床病理参数与 PD-L1 表达或 CD8 密度无关。高 CD8 密度与良好的 OS (P = .02) 和 DSS (P = .02) 相关。当根据人口统计学和临床病理学参数调整 CD8 密度时，情况也是如此。 PD-L1 表达与结果之间没有相关性。在膀胱浸润性尿路上皮癌中，肿瘤内高 CD8+ T 细胞密度与更好的 OS 和 DSS 相关。我们发现 PD-L1 表达与结果之间没有相关性。
To evaluate programmed death ligand 1 (PD-L1) expression in urothelial carcinoma of the bladder in relationship with tumor-infiltrating CD8+ T cells. Tissue microarrays were prepared from 56 cystectomy specimens performed at our hospital (1994–2002). PD-L1 immunoexpression was assessed using the murine antihuman PD-L1 monoclonal antibody 5H1. Extent of membranous PD-L1 expression was assigned in each spot. Spots showing ≥5% expression were considered positive. Average PD-L1 expression per tumor was also calculated (5% positivity cutoff). “High CD8 density” was defined as the presence of ≥60 CD8+ intraepithelial lymphocytes per high power field in a given spot. A tumor was considered high density if ≥50% of its spots were of high density. PD-L1 expression was positive in approximately 20% of tumors. None of the benign urothelium spots expressed PD-L1. High CD8 density was observed in approximately 20% of cases. CD8 density did not correlate with PD-L1 expression. Overall survival (OS) and disease-specific survival (DSS) rates were 14% and 28%, respectively (median follow-up, 31.5 months). PD-L1 expression was associated with age at cystectomy (P = .01). Remaining clinicopathologic parameters were not associated with PD-L1 expression or CD8 density. High CD8 density was associated with favorable OS (P = .02) and DSS (P = .02). The same was true when CD8 density was adjusted for demographic and clinicopathologic parameters. There was no correlation between PD-L1 expression and outcome. High intratumoral CD8+ T cell density is associated with better OS and DSS in invasive urothelial carcinoma of the bladder. We found no correlation between PD-L1 expression and outcome.