DNA Methylation of Hepatitis B Virus (HBV) Genome Associated with the Development of Hepatocellular Carcinoma and Occult HBV Infection
DNA Methylation of Hepatitis B Virus (HBV) Genome Associated with the Development of Hepatocellular Carcinoma and Occult HBV Infection
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DOI：
10.1086/655398
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发表时间：
2010-09-01
影响因子：
6.4
通讯作者：
Herceg, Zdenko
中科院分区：
文献类型：
--
作者：
Kaur, Pushpinder;Paliwal, Anupam;Herceg, Zdenko
最近的研究证实了乙肝病毒基因组中存在甲基化，并提示它可能是调节乙肝病毒转录和复制的重要机制。然而，目前尚不清楚这种现象是否与隐匿性肝炎有关。在这里，我们对处于不同发展阶段的肝癌患者的肝脏样本和体外感染的肝细胞中的乙肝病毒基因组DNA甲基化进行了详细的分析，发现在乙肝病毒基因组中的离散CpG位点在癌症组织中反复出现高甲基化，但在慢性肝炎组织中不出现。
Recent studies have identified the presence of methylation in the hepatitis B virus (HBV) genome and have suggested that it may be an important mechanism regulating transcription and replication of the HBV virus. However, it remains unclear whether this phenomenon is associated with occult hepatitis. Here, we performed a detailed analysis of DNA methylation in the HBV genome in liver samples of patients at different stages of hepatocarcinoma development and in in vitro infected hepatocytes and found discrete CpG sites in the HBV genome that are recurrently hypermethylated in cancer but not in chronic hepatitis tissue.