Effect of molybdenum on reproductive function of male mice treated with busulfan
Effect of molybdenum on reproductive function of male mice treated with busulfan
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DOI：
10.1016/j.theriogenology.2018.12.002
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发表时间：
2019-03-01
期刊：
影响因子：
2.8
通讯作者：
Zhang, Yu-Ling
中科院分区：
文献类型：
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作者：
Liu, Feng-Jun;Dong, Wen-Ya;Zhang, Yu-Ling
不孕症是一个严重的全球性公共卫生问题。钼（Mo）在维持正常代谢中起着重要作用。为探讨钼（Mo）对雄性不育的治疗作用，将90只小鼠随机分为对照组、白消安组和白消安+ Mo组。白消安组和白消安+钼组雄性小鼠腹腔注射白消安（20 mg/kg体重）建立不育模型。在白消安暴露后30天成功获得不育小鼠。白消安+钼组雄性小鼠连续饮用含钼20 mg/L的水42 d。给药后72 d，对3组中30只小鼠进行各项指标检测，并对60只小鼠进行自然发情交配。钼使细曲细精管变细，细曲细精管和生殖细胞消失。Mo还使先前异常的睾酮、雌二醇、黄体生成素、超氧化物歧化酶、乳酸脱氢酶、丙二醛水平正常化。Bax、Bcl-2、caspase-3和caspase-9的表达水平均恢复到对照组水平，最终获得数量和性别比例正常的子代。结果提示，20 mg/L钼对不育小鼠生殖功能障碍有明显的治疗作用。其机制可能是通过修复受损的睾丸结构、调节异常的生殖激素水平、降低氧化应激或抵抗细胞凋亡。Mo有望成为治疗男性不育症的一种新的候选药物。(C)2018爱思唯尔公司All rights reserved.
Infertility is a serious public health problem worldwide. Molybdenum (Mo) plays an important role in maintaining normal metabolism. To explore the therapeutic efficacy of molybdenum (Mo) on male infertility, 90 mice were randomly divided into control, busulfan and busulfan + Mo groups. The male mice in the busulfan and busulfan + Mo groups were exposed to busulfan (20 mg/kg body weight) with a single intraperitoneal injection to establish the infertility model. The sterile mice were successfully obtained 30 days after busulfan exposure. Then, the male mice in the busulfan + Mo group were given drinking water containing 20 mg/L Mo continuously for 42 days. At 72 Day after treatment, 30 mice in the three groups were tested for various indices, and 60 mice were mated with females in spontaneous estrus. Mo significantly reversed the thinner seminiferous tubules and disappeared tubule and germ cells. Mo also normalized previously abnormal levels of testosterone, estradiol, luteinizing hormone, superoxide dismutase, lactate dehydrogenase, malondialdehyde. Furthermore, expression levels expression of Bax, Bcl-2, caspase-3 and caspase-9 returned to control levels; and finally, Mo-treated sterile mice obtained offspring with normal number and gender ratio. These results suggested that Mo at 20 mg/L had a significant therapeutic effect on reproductive dysfunction in sterile mice. Its mechanism could via repair of damaged testicular structures, regulation of abnormal reproductive hormone levels, decreased oxidative stress or and resistance to cell apoptosis. Mo may be a new candidate medicine for treatment of male infertility. (C) 2018 Elsevier Inc. All rights reserved.