High Temperature Causes Breakdown of S Haplotype-Dependent Stigmatic Self-Incompatibility in Self-Incompatible Arabidopsis Thaliana
High Temperature Causes Breakdown of S Haplotype-Dependent Stigmatic Self-Incompatibility in Self-Incompatible Arabidopsis Thaliana
复制标题
高温导致自交不亲和拟南芥中 S 单倍型依赖性柱头自交不亲和的破坏
DOI：
10.1093/jxb/erz343
复制
发表时间：
2019
影响因子：
6.9
通讯作者：
Nishio T.
中科院分区：
文献类型：
--
作者：
Yamamoto M;Nishimura K;Kitashiba H;Sakamoto W;Nishio T.
十字花科蔬菜作物的商业种子大多是F1杂种，其生产依赖于授粉过程中的自交不亲和性。众所周知，暴露在高温下会削弱自交不亲和性，这可能会损害未来的育种和种子生产。在十字花科植物中，自交不亲和性由两个基因SRK和SCR控制，这两个基因分别充当识别特异性的雌性和雄性决定因素。然而，人们对高温下自不相容性破坏的分子机制知之甚少。在这项研究中，我们检查了自交不亲和拟南芥SRK-SCR转化子在正常（23°C）和升高（29°C）温度下的自交不亲和表型。暴露于高温会引起柱头缺陷，但不会引起花粉自交不亲和反应。此外，在不同的单倍型之间观察到对高温的反应存在差异。亚细胞定位表明高温破坏了 SRK 向质膜的靶向。不同单倍型转化植物中的 SRK 定位与其自交不亲和表型相对应，进一步表明 SRK 定位缺陷是高温下自交不亲和反应崩溃的原因。我们的结果为自交不亲和表型不稳定的原因提供了新的见解。
Commercial seeds of Brassicaceae vegetable crops are mostly F1hybrids, the production of which depends on self-incompatibility during pollination. Self-incompatibility is known to be weakened by exposure to elevated temperatures, which may compromise future breeding and seed production. In the Brassicaceae, self-incompatibility is controlled by two genes,SRKandSCR, which function as female and male determinants of recognition specificity, respectively. However, the molecular mechanisms underlying the breakdown of self-incompatibility under high temperature are poorly understood. In this study, we examined the self-incompatibility phenotypes of self-incompatibleArabidopsis thaliana SRK-SCRtransformants under normal (23 °C) and elevated (29 °C) temperatures. Exposure to elevated temperature caused defects in the stigmatic, but not the pollen, self-incompatibility response. In addition, differences in the response to elevated temperature were observed among differentShaplotypes. Subcellular localization revealed that high temperature disrupted the targeting of SRK to the plasma membrane. SRK localization in plants transformed with differentShaplotypes corresponded to their self-incompatibility phenotypes, further indicating that defects in SRK localization were responsible for the breakdown in the self-incompatibility response at high temperature. Our results provide new insights into the causes of instability in self-incompatibility phenotypes.