Expression of fungal pectin methylesterase in transgenic tobacco leads to alteration in cell wall metabolism and a dwarf phenotype
Expression of fungal pectin methylesterase in transgenic tobacco leads to alteration in cell wall metabolism and a dwarf phenotype
复制标题
DOI：
10.1016/j.jbiotec.2004.04.015
复制
发表时间：
2004-08-05
影响因子：
4.1
通讯作者：
Kobayashi, A
中科院分区：
文献类型：
--
作者：
Hasunuma, T;Fukusaki, E;Kobayashi, A
以转基因烟草（Nicotiana tabacum L.）产生了表达来源于黑色丝状真菌黑曲霉的真菌果胶甲基酯酶（PME; EC 3.1.1.11）基因的菌株。与植物果胶酶相比，真菌果胶酶对底物果胶的适应范围更广。正如预期的那样，在转基因烟草中果胶中甲酯的比例降低。因此，转基因植株表现出短节间、小叶片和矮化表型。在细胞水平上，茎表皮细胞的纵向长度短于对照植物。这是首次报道真菌PME促进植物矮化。值得注意的是，在表达PME的矮化植物中，细胞壁代谢相关基因包括内切-1，4-β-葡聚糖酶、纤维素合成酶、内切木葡聚糖转移酶和膨胀蛋白基因的表达水平降低。这些结果表明，真菌PME在植物中的表达影响细胞壁代谢。(C)2004 Elsevier B. V.保留所有权利。
A transgenic tobacco plant (Nicotiana tabacum L.) expressing a fungal pectin methylesterase (PME; EC 3.1.1.11) gene derived from a black filamentous fungus, Aspergillus niger was created. Fungal PME should have a wider range of adaptability to substrate pectin compared with plant PME. As expected, the proportion of methyl esters in pectin was reduced in the transgenic tobacco. Consequently, the transgenic plant showed short internodes, small leaves and a dwarf phenotype. At a cellular level, the longitudinal lengths of stem epidermal cells were shorter than those of control plants. This is the first report that fungal PME promotes dwarfism in plants. It is worth noting that in the PME-expressing dwarf plant, the expression levels of cell wall metabolism related genes that included endo-1,4-beta-glucanase, cellulose synthase, endo-xyloglucan transferase and expansin gene were decreased. These results suggest that the expression of fungal PME in plants affects the cell wall metabolism. (C) 2004 Elsevier B.V. All rights reserved.