Successful multiresistant community-associated methicillin-resistant Staphylococcus aureus lineage from Taipei, Taiwan, that carries either the novel staphylococcal chromosome cassette mec (SCCmec) type VT or SCCmec type IV
Successful multiresistant community-associated methicillin-resistant Staphylococcus aureus lineage from Taipei, Taiwan, that carries either the novel staphylococcal chromosome cassette mec (SCCmec) type VT or SCCmec type IV
复制标题
DOI：
10.1128/jcm.43.9.4719-4730.2005
复制
发表时间：
2005-09-01
影响因子：
9.4
通讯作者：
Daum, RS
中科院分区：
文献类型：
--
作者：
Boyle-Vavra, S;Ereshefsky, B;Daum, RS
耐甲氧西林金黄色葡萄球菌 (MRSA) 分离株在称为葡萄球菌染色体盒 mec (SCCmec) 的水平转移遗传元件上携带甲氧西林抗性基因 (mecA)。社区获得性 MRSA (CAMRSA) 分离株通常携带 SCCmec IV 型。我们之前报道，从台北皮肤和软组织感染 (SSTI) 儿科患者获得的 17 株 CAMRSA 分离株（多位点序列 59 型）中，76% 不携带 I 型至 IV 型 SCCmec。我们使用 DNA 序列分析来确定这些不可分型分离株所携带的元件是 SCCmec V 的一种新亚型，称为 SCCmec V.,。它包含 ccrC 重组酶基因变体 (ccrC2) 和 mec 复合物 C2。一种 SSTI 分离株包含 SCCmec IV 的分子特征，但也包含 ccrC2（SCCmec V-T 的一个特征），表明它可能含有复合 SCCmec 元件。编码 Panton-Valentine 杀白细胞素 (PVL) 的基因 lukS-PV 和 lukF-PV 存在于所有 CAMRSA SSTI 分离株中，无论它们含有 SCCmec IV 型还是 VT 型。 SCCmec V 也存在于从台北缺乏 MRSA 危险因素的健康儿童中收集的 34 个 CAMRSA 定植分离株中的 5 个（14.7%）中。这些分离株中的 4 个 (80%) 含有 lukS-PV 和 lukF-PV，27 个 (3.7%) 含有 SCCmec IV 定植分离株中的 I 个也含有 lukS-PV 和 lukF-PV。总共 63%（16 株中的 10 株）的 SSTI 分离株和 61.7%（34 株中的 21 株）测试的定植分离株对至少四类非对内酰胺类抗菌剂具有耐药性。 SCCmec VT 是一种新型 SCCmec 变体，在台北的序列类型 59 的多重抗性 CAMRSA 菌株中发现，与 PVL 白细胞毒素基因相关。
Methicillin-resistant Staphylococcus aureus (MRSA) isolates carry the methicillin resistance gene (mecA) on a horizontally transferred genetic element called the staphylococcal chromosome cassette mec (SCCmec). Community-acquired MRSA (CAMRSA) isolates usually carry SCCmec type IV. We previously reported that 76% of 17 CAMRSA isolates (multilocus sequence type 59) obtained from pediatric patients with skin and soft tissue infections (SSTI) from Taipei did not carry SCCmec types I to IV. We used DNA sequence analysis to determine that the element harbored by these nontypeable isolates is a novel subtype of SCCmec V called SCCmec V.,. It contains a ccrC recombinase gene variant (ccrC2) and mec complex C2. One SSTI isolate contained molecular features of SCCmec IV but also contained ccrC2 (a feature of SCCmec V-T), suggesting that it may harbor a composite SCCmec element. The genes lukS-PV and lukF-PV encoding the Panton-Valentine leukocidin (PVL) were present in all CAMRSA SSTI isolates whether they contained SCCmec type IV or VT. SCCmec V, was also present in 5 of 34 (14.7%) CAMRSA colonization isolates collected from healthy children from Taipei who lacked MRSA risk factors. Four (80%) of the these isolates contained lukS-PV and lukF-PV, as did I of 27 (3.7%) SCCmec IV-containing colonization isolates. A total of 63% (10 of 16) of the SSTI isolates and 61.7% (21 of 34) of the colonization isolates tested were resistant to at least four classes of non-p-lactam antimicrobials. SCCmec VT is a novel SCCmec variant that is found in multiply resistant CAMRSA strains with sequence type 59 in Taipei in association with the PVL leukotoxin genes.