SAMPLING MICROBIAL VOLATILE ORGANIC COMPOUNDS: OPTIMISATION OF FLOW RATE AND SAMPLING TIME
SAMPLING MICROBIAL VOLATILE ORGANIC COMPOUNDS: OPTIMISATION OF FLOW RATE AND SAMPLING TIME
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DOI：
10.2495/air170071
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发表时间：
2017-04
影响因子：
12
通讯作者：
Sonia Garcia Alcega;S. Tyrrel;F. Coulon
中科院分区：
文献类型：
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作者：
Sonia Garcia Alcega;S. Tyrrel;F. Coulon
城市、农业和工业环境排放的生物气溶胶对当地空气质量的影响日益受到政策关注。然而，尽管使用了大量的生物气溶胶采样方法，但尚未建立标准化的方案。此外，捕获足够数量的物质以允许可重复的分离和分子模式检测仍然是困难的。微生物挥发性有机化合物（MVOC）的化学指纹分析是一种潜在的快速和可复制的方法，可用于室外污染的早期检测和鉴定，因为它已被证明是一种成功的室内环境方法，并且可以在精细尺度上完成，允许鉴定物种特异性挥发物，这些挥发物可能作为选择性检测病原体的标记化合物。在本研究中，我们测试了不同采样条件下收集的MVOCs的数量和浓度：10分钟采样时间，可变流量（100、500和1000 ml min - 1）和100 ml min - 1流量，10,20和30分钟，使用Tenax®-Carbotrap热解吸（TD）管连接到便携式GilAir®气泵。我们的目的是确定最佳采样条件，以便获得足够的材料，允许在室外环境中存在的微生物标记物的可重复数据。当流速高于100 ml min-1时，MVOCs的大量损失（bbb50 %）发生。10 min的采样时间可以收集到空气中大部分的MVOCs（~96%）。最佳采样设置为以100 ml min - 1流速采样10 min，可以在不突破的情况下收集较高浓度的MVOCs。其中，酮类化合物含量最高（34-42%），丙酮含量最高（6476 ~ 11731 ng m-3）。
The impact of bioaerosols emissions from urban, agricultural and industrial environments on local air quality is of growing policy concern. However, there is no standardised protocol established yet, despite a large number of bioaerosols sampling methods in use. Additionally, capturing sufficient amounts of material to allow reproducible separation and detection of molecular patterns is still difficult. Chemical fingerprint analysis of microbial volatile organic compounds (MVOC) is a potentially rapid and reproducible approach for the early detection and identification of outdoor contamination as it has been shown to be a successful approach for indoor environments and it can be done on a fine-scale, allowing the identification of species-specific volatiles that may serve as marker compounds for the selective detection of pathogens. In this study we have tested the number and concentration of MVOCs collected using different sampling conditions: 10 min sampling time with variable flow rate (100, 500 and 1000 ml min–1) and 100 ml min–1 flow rate during 10, 20 and 30 min using Tenax®-Carbotrap thermal desorption (TD) tubes attached to portable GilAir® air pumps. Our aim was to determine the best sampling conditions in order to get enough material allowing reproducible data of the microbial markers present in outdoor environments. Substantial loses (>50%) of MVOCs occurred when sampling at flow rates higher than 100 ml min–1. 10 min sampling time allowed the collection of most of the MVOCs present in the air (~96%). The optimal sampling settings that allowed the collection of higher concentrations of MVOCs without breakthrough was 10 min sampling at 100 ml min–1 flow rate. Ketones were the predominant group of MVOCs identified in the WWTP (34–42%), acetone being the compound present at higher concentration (6476–11731 ng m–3).