INTERACTIONS OF MAMMALIAN-CELLS WITH LIPID DISPERSIONS CONTAINING NOVEL METABOLIZABLE CATIONIC AMPHIPHILES
INTERACTIONS OF MAMMALIAN-CELLS WITH LIPID DISPERSIONS CONTAINING NOVEL METABOLIZABLE CATIONIC AMPHIPHILES
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DOI：
10.1016/0005-2736(90)90017-i
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发表时间：
1990-03-30
期刊：
影响因子：
--
通讯作者：
SILVIUS, JR
中科院分区：
文献类型：
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作者：
LEVENTIS, R;SILVIUS, JR
几种新型的阳离子两亲物，基于疏水胆固醇或二油酰甘油基部分，已被制备，其疏水和阳离子部分通过酯键连接，以促进在动物细胞中的有效降解。将此类阳离子物质与磷脂酰乙醇胺（PE）、PE的某些结构类似物或二酰基甘油组合的分散体可以介导不可交换的脂质探针和DNA质粒pSV 2cat有效转移到培养的哺乳动物（CV-1和3 T3）细胞中。不同类型的阳离子脂质分散体介导用pSV 2cat转染哺乳动物细胞的能力不能与它们与其他膜聚结的能力直接相关，如通过它们在存在或不存在DNA的情况下有效地将脂质与大的单层磷脂酰胆碱/磷脂酰丝氨酸囊泡混合的能力所评估的。对CV-1细胞的细胞毒性的分散体相结合的PE与大多数的可降解的阳离子两亲物在这里研究相比，有利的与以前报道的类似的分散体含有其他类型的阳离子两亲物。在这项研究中检查的两个基于二酰基甘油的阳离子两亲物的荧光类似物被证明是容易降解后，纳入CV-1细胞从PE/阳离子脂质分散体。
Several novel cationic amphiphiles, based on a hydrophobic cholesteryl or dioleoylglyceryl moiety, have been prepared whose hydrophobic and cationic protions are linked by ester bonds to facilitate efficient degradation in animal cells. Dispersions combining such cationic species with phosphatidylethanolamine (PE), certain structural analogues of PE or diacylglycerol can mediate efficient transfer of both nonexchangeable lipid probes and the DNA plasmid pSV2cat into culture mammalian (CV-1 and 3T3) cells. The abilities of different types of cationic lipid dispersions to mediate transfection of mammalian cells with pSV2cat could not be directly correlated with their abilities to coalesce with other membranes, as assessed by their ability to intermix lipids efficiently with large unilamellar phosphatidylcholine/phosphatidylserine vesicles in the presence or absence of DNA. The cytotoxicities toward CV-1 cells of dispersions combining PE with most of the degradable cationic amphiphiles studied here compare favorably with those reported previously for similar dispersions containing other types of cationic amphiphiles. Fluorescent analogues of two of the diacylglycerol-based cationic amphiphiles examined in this study are shown to be readily degraded after incorporation into CV-1 cells from PE/cationic lipid dispersions.