BRYOSTATINS - POTENT, NEW MITOGENS THAT MIMIC PHORBOL ESTER TUMOR PROMOTERS
BRYOSTATINS - POTENT, NEW MITOGENS THAT MIMIC PHORBOL ESTER TUMOR PROMOTERS
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DOI：
10.1016/0006-291x(85)91898-4
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
PETTIT, GR
中科院分区：
文献类型：
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作者：
SMITH, JB;SMITH, L;PETTIT, GR
苔藓抑素（2ng /ml）与胰岛素在无血清培养基中联合使用时，对处于细胞周期G1/G0期的Swiss 3T3细胞具有强烈的促丝分裂作用。苔藓抑素的有丝分裂作用与12-0-癸烷酰磷-13-乙酸酯（TPA）相似。先前用TPA处理的培养物消除了对苔藓虫素和第二次添加TPA的有丝分裂反应。相反，事先用苔藓虫素处理的培养物消除了对TPA的有丝分裂反应。苔藓虫素能有效抑制[3H]二丁酸磷与细胞内高亲和力受体的结合。研究结果表明苔藓抑素和TPA通过相同的受体起作用，可能是蛋白激酶C。
Bryostatins (2 ng/ml), when combined with insulin in serum-free culture medium, are strongly mitogenic for Swiss 3T3 cells that have been arrested in the G1/G0 phase of the cell cycle. The mitogenic effect of the bryostatins is similar to that of 12-0-decanoylphorbol-13-acetate (TPA). A prior treatment of the cultures with TPA eliminated the mitogenic response to bryostatin and to a second addition of TPA. Conversely, a prior treatment of the cultures with bryostatin eliminated the mitogenic response to TPA. Bryostatin potently inhibited the binding of [3H]phorbol dibutyrate to a high affinity receptor in the cells. The findings suggest that the bryostatins and TPA act via the same receptor, possibly protein kinase C.