Differential regulation of spleen cell-mediated eosinophil and neutrophil-macrophage production.
Differential regulation of spleen cell-mediated eosinophil and neutrophil-macrophage production.
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脾细胞介导的嗜酸性粒细胞和中性粒细胞巨噬细胞产生的差异调节。
DOI：
10.1182/blood.v55.3.489.bloodjournal553489
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发表时间：
1980
期刊：
影响因子：
20.3
通讯作者：
D. Bass
中科院分区：
文献类型：
--
作者：
S. Bartelmez;W. Dodge;D. Bass
旋毛虫感染小鼠的非贴壁脾细胞在体外释放生长刺激因子（GSFs）时，肌肉期幼虫的排泄/分泌产物的挑战。GSF的测定是基于液体管培养中正常、非贴壁的同源骨髓细胞的增殖。经激发的脾细胞调节1天的培养基刺激嗜酸性粒细胞的产生，但未能刺激其他细胞类型的产生。相比之下，条件化5天的培养基支持嗜酸性粒细胞、嗜中性粒细胞和巨噬细胞的产生。细胞生产的动力学也不同。嗜酸性粒细胞的产生在1天内开始，在第2天达到峰值，并在第4天降至对照水平。相比之下，中性粒细胞/巨噬细胞的产生开始于2 - 4天，并在6- 8天达到高峰。嗜酸性粒细胞产生的短持续时间显然是由于生长因子反应细胞的耗竭。
Nonadherent spleen cells of mice infected with Trichinella spiralis released growth stimulatory factors (GSFs) in vitro when challenged with excretory/secretory products of muscle stage larvae. The assay of GSF was based on proliferation of normal, nonadherent syngeneic marrow cells in liquid tube cultures. Media conditioned for 1 day by challenged spleen cells stimulated eosinophil production but failed to stimulate production of other cell types. In contrast, media conditioned for 5 days supported eosinophil, neutrophil, and macrophage production. The kinetics of cell production were also different. Eosinophil production started within 1 day, reached a peak at day 2, and was down to control levels by day 4. In contrast, neutrophil/macrophage production began between 2 and 4 days and reached a peak at 6--8 days. The short duration of eosinophil production was evidently due to depletion of growth-factor-responsive cells.