The salivary microbiota as a diagnostic indicator of oral cancer: A descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects
The salivary microbiota as a diagnostic indicator of oral cancer: A descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects
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DOI：
10.1186/1479-5876-3-27
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发表时间：
2005-07-07
影响因子：
7.4
通讯作者：
Goodson, JM
中科院分区：
文献类型：
--
作者：
Mager, DL;Haffajee, AD;Goodson, JM
背景：本研究的目的是确定患有口腔鳞状细胞癌 (OSCC) 病变的受试者中 40 种常见口腔细菌的唾液计数是否与无癌（无 OSCC）对照中的唾液计数不同。方法：从 229 名无 OSCC 受试者和 45 名 OSCC 受试者中收集未刺激的唾液样本，并使用棋盘 DNA-DNA 杂交评估其 40 种常见口腔细菌的含量。确定每个物种每毫升唾液的 DNA 计数，对 2 个受试者组中的受试者进行平均，并使用曼-惠特尼检验确定组间差异的显着性，并针对多重比较进行调整。通过唾液微生物水平计算检测 OSCC 的诊断敏感性和特异性，并分别在由 45 名 OSCC 受试者和 229 名对照组成的非匹配组与由 45 名 OSCC 受试者和 45 名年龄、性别和吸烟史匹配的对照组成的组之间分别进行比较。 结果：测试的 40 个物种中的 3 个物种（牙龈二氧化碳嗜纤维菌、产黑色素普雷沃氏菌和轻症链球菌）的计数在OSCC 患者的唾液 (p < 0.001)。当作为诊断标记物进行测试时，发现这 3 个物种可以预测 80% 的癌症病例（敏感性），同时排除非匹配组中 83% 的对照（特异性）。对照组的诊断敏感性和特异性分别为80%和82%。结论：唾液中高的牙龈C. gingivalis、P. melaninogenica和S. mitis计数可能是OSCC的诊断指标。
Background: The purpose of the present investigation was to determine if the salivary counts of 40 common oral bacteria in subjects with an oral squamous cell carcinoma (OSCC) lesion would differ from those found in cancer-free (OSCC- free) controls.Methods: Unstimulated saliva samples were collected from 229 OSCC-free and 45 OSCC subjects and evaluated for their content of 40 common oral bacteria using checkerboard DNA-DNA hybridization. DNA counts per ml saliva were determined for each species, averaged across subjects in the 2 subject groups, and significance of differences between groups determined using the Mann-Whitney test and adjusted for multiple comparisons. Diagnostic sensitivity and specificity in detection of OSCC by levels of salivary organisms were computed and comparisons made separately between a non-matched group of 45 OSCC subjects and 229 controls and a group of 45 OSCC subjects and 45 controls matched by age, gender and smoking history.Results: Counts of 3 of the 40 species tested, Capnocytophaga gingivalis, Prevotella melaninogenica and Streptococcus mitis, were elevated in the saliva of individuals with OSCC (p < 0.001). When tested as diagnostic markers the 3 species were found to predict 80% of cancer cases (sensitivity) while excluding 83% of controls (specificity) in the non-matched group. Diagnostic sensitivity and specificity in the matched group were 80% and 82% respectively.Conclusion: High salivary counts of C. gingivalis, P. melaninogenica and S. mitis may be diagnostic indicators of OSCC.