Survival of nigral grafts within the striatum of marmosets with 6-OHDA lesions depends critically on donor embryo age
Survival of nigral grafts within the striatum of marmosets with 6-OHDA lesions depends critically on donor embryo age
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DOI：
10.1016/s0963-6897(97)00079-1
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发表时间：
1997-11-01
影响因子：
3.3
通讯作者：
Dunnett, SB
中科院分区：
文献类型：
--
作者：
Annett, LE;Torres, EM;Dunnett, SB
该研究探讨了胚胎供体年龄对6-OHDA损伤的绒猴黑质移植物存活的重要性。关于供体年龄是否对灵长类动物中黑质移植物的存活至关重要的问题是有争议的，因为一些早期的报告表明，相对较老的组织可以在移植后存活并在猴子中产生功能益处，而不是在啮齿动物中观察到的限制性时间依赖性。使用三种不同年龄的绒猴胚胎供体胚胎：1）E74（Carnegie阶段18-19）; 2）E83-84（Carnegie阶段23+）; 3）E92-93（胎儿期）。黑质神经元来自腹侧中脑在两个年龄较大的捐助者年龄组没有存活良好时，移植到成年绒猴纹状体单侧6-OHDA病变。虽然一些酪氨酸羟化酶（TH+）神经元可以通过免疫组织化学在移植部位在所有受体在老年供体年龄组，这些存活的神经元的数量很少，平均通常小于100 TH+细胞。这些小移植物不足以影响安非他明诱导的旋转。相比之下，更多的TH+细胞通常存活移植在受体的移植组织来源于最年轻的供体和安非他明诱导的旋转显着减少，在这一组单独。旋转减少的时间过程和程度与之前的绒猴黑质移植研究中观察到的结果非常相似，证实了安非他明诱导的旋转作为该物种黑质移植功能的敏感且可靠的指标的实用性。考虑到这些结果和其他最近从猴到猴、人到大鼠和人到人的移植研究的证据，移植到纹状体中的灵长类动物供体的胚胎黑质组织的存活似乎确实严重依赖于供体组织的年龄。(C)1997年爱思唯尔科学公司
The study examined the importance of embryonic donor age for the survival of nigral grafts in 6-OHDA-lesioned marmosets. The issue as to whether donor age is critical for the survival of nigral grafts in primates is controversial, because several early reports suggested that relatively old tissue could survive transplantation and produce functional benefits in monkeys, in contrast to the restrictive time dependence observed in rodents. Embryonic marmoset donors embryos of three different ages were employed: 1) E74 (Carnegie stage 18-19); 2) E83-84 (Carnegie stage 23+); 3) E92-93 (foetal period). The nigral neurons derived from the ventral mesencephalon in the two older donor age groups did not survive well when grafted to the striatum of adult marmosets with unilateral 6-OHDA lesions. Although a few tyrosine hydroxylase (TH+) neurons could be identified by immunohistochemistry at graft sites in all recipients in older donor age groups, the numbers of surviving neurons in these were small, on average typically less than 100 TH+ cells. These small grafts were not sufficient to affect amphetamine-induced rotation. In contrast, many more TH+ cells typically survived transplantation In the recipients of graft tissue derived from the youngest donors and amphetamine-induced rotation was significantly reduced in this group alone. The time course and extent of the reduction in rotation was remarkably similar to that observed in previous marmoset nigral graft studies, confirming the utility of amphetamine-induced rotation as a sensitive and reliable indicator of nigral graft function in this species. Considering these results and other recent evidence from monkey to monkey, human to rat, and human to human graft studies, the survival of embryonic nigral tissues derived from primate donors transplanted into the striatum does appear to be critically dependent on the age of the donor tissue. (C) 1997 Elsevier Science Inc.