5-HT1A receptor agonist reverses adrenalectomy-induced loss of granule neuronal morphology in the rat dentate gyrus.
5-HT1A receptor agonist reverses adrenalectomy-induced loss of granule neuronal morphology in the rat dentate gyrus.
复制标题
5-HT1A 受体激动剂可逆转肾上腺切除术引起的大鼠齿状回颗粒神经元形态的丧失。
DOI：
10.1023/a:1022062921438
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发表时间：
1997
影响因子：
4.4
通讯作者：
Azmitia,EC
中科院分区：
文献类型：
--
作者：
Huang,J;Strafaci,JA;Azmitia,EC
肾上腺皮质类固醇对维持成年大鼠神经元的成熟具有重要作用。双侧肾上腺切除（ADX）后2周，海马齿状回上级叶分子层微管相关蛋白2（MAP-2）和钙结合蛋白免疫反应性（IR）降低。上级叶片侧端的分子细胞层和颗粒细胞层的宽度分别减少了32%和50%。颗粒神经元显示尼氏染色和抗钙结合蛋白抗体减少。这些变化表明成熟神经元形态的丧失。在同一区域，两种胶质蛋白，胶质细胞酸性蛋白（GFAP）和S-100β的免疫反应性显着降低。5 HT 1A受体激动剂ipsapiron 1 mg/kg可在72小时内降低ADX诱导的上述效应。ADX导致的成年神经元形态学的丧失和5 HT 1A激动剂的逆转可能是海马颗粒神经元中5 HT 1A受体营养重要性的证据。
Adrenal steroids are important for maintaining neuronal maturation in the adult rats. Two weeks after bilateral adrenalectomy (ADX), hippocampal MAP-2 (microtubule associated protein-2) and calbindin immunoreactivity (IR) decreased in the molecular layer of the superior blade of the dentate gyrus. The molecular and granular cell layer at the lateral tip of the superior blade decreased in width by 32% and 50%, respectively. The granule neurons showed reduced staining with Nissl and an anti-calbindin antibody. These changes suggested a loss of the mature neuronal morphology. In this same localized regions, two glial proteins, glial fibrillary acidic protein (GFAP) and S-100β showed dramatically reduced immunoreactivity. These effects induced by ADX were reduced within 72 hrs by ipsapirone (1 mg/kg), a 5HT1Areceptor agonist. Loss of adult neuronal morphology by ADX, and reversal by the 5HT1Aagonist, may be evidence of the trophic importance of the 5HT1Areceptor in granule neurons of hippocampus.