The heat-shock element is a functional component of the Arabidopsis APX1 gene promoter.
The heat-shock element is a functional component of the Arabidopsis APX1 gene promoter.
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DOI：
10.1104/pp.118.3.1005
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发表时间：
1998-11
期刊：
影响因子：
7.4
通讯作者：
S. Storozhenko;Pascal De Pauw;M. V. Montagu;Dirk Inzé;S. Kushnir
中科院分区：
文献类型：
--
作者：
S. Storozhenko;Pascal De Pauw;M. V. Montagu;Dirk Inzé;S. Kushnir
抗坏血酸过氧化物酶是植物细胞质和叶绿体中解毒过氧化氢的重要酶。为了更好地了解它们在氧化应激耐受中的作用，研究了拟南芥apx1基因的转录调控。apx1基因在拟南芥所有器官中均有表达；mRNA水平在根、叶和茎中较低，在花中较高。黄化幼苗经紫甲基、乙烯利、高温和光照处理后，叶片或细胞悬液中稳态mRNA水平升高。在apx1启动子中发现的一个假定的热休克顺式元件在体外被番茄（Lycopersicon esculentum）热休克因子识别，并负责基因的体内热休克诱导。热休克顺式因子也对氧化应激诱导该基因有部分贡献。通过在体内使用硫酸二甲酯足迹，我们发现蛋白质与apx1启动子中发现的富含G/ c的元素相互作用。
Ascorbate peroxidases are important enzymes that detoxify hydrogen peroxide within the cytosol and chloroplasts of plant cells. To better understand their role in oxidative stress tolerance, the transcriptional regulation of the apx1 gene from Arabidopsis was studied. The apx1 gene was expressed in all tested organs of Arabidopsis; mRNA levels were low in roots, leaves, and stems and high in flowers. Steady-state mRNA levels in leaves or cell suspensions increased after treatment with methyl viologen, ethephon, high temperature, and illumination of etiolated seedlings. A putative heat-shock cis element found in the apx1 promoter was shown to be recognized by the tomato (Lycopersicon esculentum) heat-shock factor in vitro and to be responsible for the in vivo heat-shock induction of the gene. The heat-shock cis element also contributed partially to the induction of the gene by oxidative stress. By using in vivo dimethyl sulfate footprinting, we showed that proteins interacted with a G/C-rich element found in the apx1 promoter.