[The detection of plasmid pCAMBIA1301 in transgenic rice by arrayed primer extension].
[The detection of plasmid pCAMBIA1301 in transgenic rice by arrayed primer extension].
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发表时间：
2005-03
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通讯作者：
Xiu-Li Gao;Jian-Bo Yang;F. Jing;Jian-long Zhao
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作者：
Xiu-Li Gao;Jian-Bo Yang;F. Jing;Jian-long Zhao
生物芯片技术是20世纪80年代末生物技术与微纳生物技术相融合而产生的，目前已广泛应用于生命科学、医学、临床诊断、药物开发、农业、环境保护和战略等领域。DNA微阵列（又称基因芯片、DNA芯片）是生物芯片的一种，是含有多个寡核苷酸探针的小芯片。它可以与标记的样品杂交，使得一次检测大量寡核苷酸成为可能。因此，DNA微阵列技术可以克服传统杂交技术复杂、自动化程度低、效率低、检测分子量大等缺点。本文介绍了一种利用基因芯片检测转基因植物的新方法。我们开发了一种新的阵列引物延伸技术。它将杂交和PCR结合在一个步骤中，而普通的DNA微阵列需要两个独立的步骤。因此，我们的方法提供了一个可行的检测长DNA片段。
Biochip technology which had emerged from the fusion of biotechnology and micro/nanofabrication technology at the end of 1980s has been widely used in life science, medicine, clinical diagnosis, drug development, agriculture, environmental protection and strategies. DNA microarray (also call gene chip, DNA chip), one kind of biochips, is small chip containing many oligonucleotide probes. It can hybridize with labelled sample, making it possible to detect large numbers of oligonucleotides at one time. So DNA microarray can overcome the disadvantage of traditional hybridization technology such as complexity, low automation, poor efficiency and quantity of molecules detected. This paper describes a new method to detect transgenic plant with gene chip. We have developed a novel arrayed-primer extension technique. It combines hybridization and PCR in one step, while ordinary DNA microarray needs two separate steps. Therefore our method provide a feasibility to detect long DNA fragment .