INTERACTION OF U6 SNRNA WITH A SEQUENCE REQUIRED FOR FUNCTION OF THE NEMATODE SL RNA IN TRANSSPLICING
INTERACTION OF U6 SNRNA WITH A SEQUENCE REQUIRED FOR FUNCTION OF THE NEMATODE SL RNA IN TRANSSPLICING
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DOI：
10.1126/science.1465612
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发表时间：
1992-12-11
期刊：
影响因子：
56.9
通讯作者：
NILSEN, TW
中科院分区：
文献类型：
--
作者：
HANNON, GJ;MARONEY, PA;NILSEN, TW
线虫反式剪接前导序列（SL）RNA由两个结构域组成，一个外显子（22个核苷酸的剪接前导序列）和一个小核RNA（snRNA）样序列。剪接的前导RNA在反式剪接反应中的体外参与与外显子序列或大小无关，而是取决于分子的snRNA样结构域中所含的特征。化学修饰干扰分析表明，两个短序列元件的snRNA样结构域是必要的SL RNA活性。这些元件对于这种活性是足够的，因为当添加到线虫U1 snRNA的72个核苷酸片段时，该杂合RNA可以参与体外反式剪接反应。其中一个关键序列元件可能通过与U6 snRNA（一种顺式和反式剪接必需的U snRNA）碱基配对发挥作用。
Nematode trans-spliced leader (SL) RNAs are composed of two domains, an exon [the 22-nucleotide spliced leader] and a small nuclear RNA (snRNA)-like sequence. Participation in vitro of the spliced leader RNA in trans-splicing reactions is independent of the exon sequence or size and instead depends on features contained in the snRNA-like domain of the molecule. Chemical modification interference analysis has revealed that two short sequence elements in the snRNA-like domain are necessary for SL RNA activity. These elements are sufficient for such activity because when added to a 72-nucleotide fragment of a nematode U1 snRNA, this hybrid RNA could participate in trans-splicing reactions in vitro. One of the critical sequence elements may function by base-pairing with U6 snRNA, an essential U snRNA for both cis- and trans-splicing.