Discovery and metagenomic analysis of an anammox bacterial enrichment related to Candidatus "Brocadia caroliniensis" in a full-scale glycerol-fed nitritation-denitritation separate centrate treatment process.
Discovery and metagenomic analysis of an anammox bacterial enrichment related to Candidatus "Brocadia caroliniensis" in a full-scale glycerol-fed nitritation-denitritation separate centrate treatment process.
复制标题
DOI：
10.1016/j.watres.2017.01.011
复制
发表时间：
2017-03
期刊：
影响因子：
12.8
通讯作者：
Hongkeun Park;A. C. Brotto;M. V. van Loosdrecht;K. Chandran
中科院分区：
文献类型：
--
作者：
Hongkeun Park;A. C. Brotto;M. V. van Loosdrecht;K. Chandran
在耦合部分硝化（亚硝化）和部分反硝化（反硝化）过程的甘油进料消化池液体流出物处理工艺中观察到独特的红色生物膜。基于16 S rRNA克隆文库和定量聚合酶链反应的初步系统发育筛选，生物膜富集了与Brocadia caroliniensis密切相关的新型厌氧氨氧化细菌（AMX/anammox）。C.“Brocadia caroliniensis”富集进一步探索使用高通量测序和de novometagenome组装。从宏基因组分箱的群体anammox基因组由209个重叠群组成，共有3.73 Mbp的共有序列，具有43.3%的GC含量和27.4的平均覆盖深度。组装的宏基因组箱由3582个开放阅读框（ORF）组成。基于16 SrRNA的相似性分析，该宏基因组与Brocadia caroliniensis、Brocadia fulgida、植物真菌KSU-1和Kuenenenia stuttgartiensis的相似性分别为99%、96%、92%和93%。此外，在该菌群体基因组中还发现了氨和亚硝酸盐转运、肼合成、催化剂电子传递和无机碳固定等厌氧氨氧化代谢功能的必需基因。“Brocadia caroliniensis”相关富集。我们的研究结果表明，工程脱氮系统中的厌氧氨氧化细菌比预期的更广泛。还强调了宏基因组学方法在这些系统中破译这种新功能的实用性。
A distinctive red biofilm was observed in a glycerol-fed digester liquid effluent treatment process coupling partial nitrification (nitritation) and partial denitrification (denitritation) processes. Based on initial phylogenetic screening using 16S rRNA clone libraries and quantitative polymerase chain reaction, the biofilm was enriched in novel anaerobic ammonium oxidizing bacteria (AMX/anammox) closely related toCandidatus“Brocadia caroliniensis”. The metabolic functionality of theC.“Brocadia caroliniensis” enrichment was further explored using high-throughput sequencing andde novometagenome assembly. The population anammox genome that was binned from the metagenome consisted of 209 contigs with a total of 3.73 Mbp consensus sequences having 43.3% GC content, and 27.4 average coverage depth. The assembled metagenome bin was comprised of 3582 open reading frames (ORFs). Based on 16S rRNA similarity the binned metagenome was closely related withCandidatus“Brocadia caroliniensis”,Candidatus“Brocadia fulgida”, planctomycete KSU-1, andCandidatus“Kuenenia stuttgartiensis” with 99%, 96%, 92% and 93% similarity, respectively. Essential genes in anammox metabolic functions including ammonium and nitrite transport, hydrazine synthesis, electron transfer for catabolism, and inorganic carbon fixation, among several other anabolic pathways, were also observed in the population genome of theC. “Brocadia caroliniensis” related enrichment. Our results demonstrate the wider profusion of anammox bacteria in engineered nitrogen removal systems than expected. The utility of metagenomics approaches to deciphering such novel functionality in these systems is also highlighted.