Ethylene Signaling Modulates Cortical Microtubule Reassembly in Response to Salt Stress
Ethylene Signaling Modulates Cortical Microtubule Reassembly in Response to Salt Stress
复制标题
乙烯信号调节皮质微管重组以响应盐胁迫
DOI：
10.1104/pp.17.01124
复制
发表时间：
2018-03-01
期刊：
影响因子：
7.4
通讯作者：
Mao, Tonglin
中科院分区：
文献类型：
--
作者：
Dou, Liru;He, KaiKai;Mao, Tonglin
皮层微管重组的调节对于植物细胞在高盐条件下的存活是必不可少的。在盐胁迫下，微管经历快速解聚，然后重新组装，形成一个新的微管网络，促进细胞存活;然而，这种恢复反应的上游调控机制在很大程度上是未知的。在这项研究中，我们证明，乙烯信号促进盐胁迫诱导的重组拟南芥（拟南芥）皮层微管。微管解聚不受影响，盐胁迫下抑制乙烯信号与Ag+或乙烯不敏感的突变体，而微管重组显着抑制。ETHYLENE-INSENSITIVE3是乙烯信号转导途径中的一个关键转录因子，在盐胁迫下微管重组中起着重要作用。此外，我们进行了微管稳定蛋白WAVE-DAMPENED2-LIKE 5（WDL 5）的功能表征，发现该蛋白促进乙烯相关微管重组和植物盐胁迫耐受性。这些发现表明，乙烯信号调节微管重组上调WDL5表达响应盐胁迫，从而暗示乙烯信号在植物耐盐胁迫。
Regulation of cortical microtubule reorganization is essential for plant cell survival under high salinity conditions. In response to salt stress, microtubules undergo rapid depolymerization followed by reassembly to form a new microtubule network that promotes cell survival; however, the upstream regulatory mechanisms for this recovery response are largely unknown. In this study, we demonstrate that ethylene signaling facilitates salt stress-induced reassembly of cortical microtubules in Arabidopsis (Arabidopsis thaliana). Microtubule depolymerization was not affected under salt stress following the suppression of ethylene signaling with Ag+ or in ethylene-insensitive mutants, whereas microtubule reassembly was significantly inhibited. ETHYLENE-INSENSITIVE3, a key transcription factor in the ethylene signaling pathway, was shown to play a central role in microtubule reassembly under salt stress. In addition, we performed functional characterization of the microtubule-stabilizing protein WAVE-DAMPENED2-LIKE5 (WDL5), which was found to promote ethylene-associated microtubule reassembly and plant salt stress tolerance. These findings indicate that ethylene signaling regulates microtubule reassembly by up-regulating WDL5 expression in response to salt stress, thereby implicating ethylene signaling in salt-stress tolerance in plants.