A microwell array system for stem cell culture
A microwell array system for stem cell culture
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DOI：
10.1016/j.biomaterials.2007.10.030
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发表时间：
2008-02-01
期刊：
影响因子：
14
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Moeller, Hannes-Christian;Mian, Matthew K.;Khademhosseini, Ali
定向胚胎干细胞（ES）分化是一种潜在的强大的方法，为再生医学产生可再生细胞来源。典型的体外胚胎干细胞分化方案涉及胚胎干细胞聚集中间体的形成，称为胚状体（EBs）。最近，我们展示了使用聚乙二醇（PEG）微孔作为模板来指导这些聚集体的形成，提供对尺寸、形状和均匀性等参数的控制。尽管取得了这些有希望的结果，但先前开发的技术存在局限性，因为难以重复地获得高效和可回收的均匀EBs培养物。在这项研究中，我们通过优化许多特征来改进平台：微孔的材料组成、细胞播种程序和聚合检索方法。通过这些修改，我们证明了所得总体种群的均匀性程度得到改善，并建立了一个强大的方案，以提高EB的形成效率。优化后的微孔阵列系统是胚胎干细胞分化研究和高通量干细胞实验的潜在通用工具。(c) 2007 Elsevier Ltd.版权所有。
Directed embryonic stem (ES) cell differentiation is a potentially powerful approach for generating a renewable source of cells for regenerative medicine. Typical in vitro ES cell differentiation protocols involve the formation of ES cell aggregate intermediates called embryoid bodies (EBs). Recently, we demonstrated the use of poly(ethylene glycol) (PEG) microwells as templates for directing the formation of these aggregates, offering control over parameters such as size, shape, and homogeneity. Despite these promising results, the previously developed technology was limited as it was difficult to reproducibly obtain cultures of homogeneous EBs with high efficiency and retrievability. In this study, we improve the platform by optimizing a number of features: material composition of the microwells, cell seeding procedures, and aggregate retrieval methods. Adopting these modifications, we demonstrate an improved degree of homogeneity of the resulting aggregate populations and establish a robust protocol for eliciting high EB formation efficiencies. The optimized microwell array system is a potentially versatile tool for ES cell differentiation studies and high-throughput stem cell experimentation. (c) 2007 Elsevier Ltd. All rights reserved.