Effect of high-fat diet on body composition and hormone responses to glucose tolerance tests
Effect of high-fat diet on body composition and hormone responses to glucose tolerance tests
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DOI：
10.1385/endo:19:3:327
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发表时间：
2002-12-01
期刊：
影响因子：
3.7
通讯作者：
Spicer, LJ
中科院分区：
文献类型：
--
作者：
Brown, JL;Spicer, MT;Spicer, LJ
为了确定高脂饮食对胰岛素抵抗发展的影响的潜在激素介质，在随机分配到高脂饮食组的断奶雌性Sprague-Dawley大鼠中评价血液瘦素、生长激素（GH）、葡萄糖和胰岛素对2g/kg BW口服葡萄糖激发的反应。（HF，39%的卡路里，20%的脂肪，按重量计; n = 10）和中等脂肪（MF，22%的卡路里，10%的脂肪，按重量计; n = 10）饮食。口服葡萄糖挑战后，5，7，和9周的喂养试验。向动物提供超过其生长需求的饲料。在试验的第6、8和10周通过双能X线吸收测量法（DXA）进行体重分析。HF动物在7周后体重增加更多，具有比MF动物更大的体脂，并且对口服葡萄糖挑战的葡萄糖反应相似。HF大鼠分泌更多的胰岛素和瘦素相比，MF动物。瘦体重和血清GH和IGF-I浓度组间无差异。这项研究的结果表明，瘦素，而不是GH或IGF-I参与胰岛素抵抗的发展，在生长大鼠作为一个结果，过量的能量摄入的形式，膳食脂肪。
To determine potential hormonal mediators of the effect of high-fat diets on the development of insulin resistance, blood leptin, growth hormone (GH), glucose, and insulin responses to a 2 g/kg BW oral glucose challenge were evaluated in weanling female Sprague-Dawley rats that were randomly assigned to a high-fat (HF, 39% of calories, 20% fat by weight; n = 10) and moderate-fat (MF, 22% of calories, 10% fat by weight; n = 10) diets. Oral glucose challenges were administered following 5, 7, and 9 wk on the feeding trial. Animals were provided diet in excess of their requirements for growth. Body mass analysis was conducted by dual X-ray absorptiometry (DXA) on the 6th, 8th, and loth weeks of the trial. HF animals gained more weight after 7 wk, had greater body fat than the MF animals, and similar glucose responses to the oral glucose challenges. HF rats secreted more insulin and leptin compared to MF animals. Lean body mass and serum GH and IGF-I concentrations were not different between the groups. Results of this study demonstrate that leptin but not GH or IGF-I is involved in the development of insulin resistance in growing rats as a result of excess energy intake in the form of dietary fat.