Pathological Study of Chronic Pulmonary Toxicity Induced by Intratracheally Instilled Asian Sand Dust (Kosa)
Pathological Study of Chronic Pulmonary Toxicity Induced by Intratracheally Instilled Asian Sand Dust (Kosa)
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DOI：
10.1177/0192623312452490
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发表时间：
2013-01-01
影响因子：
1.5
通讯作者：
Takano, Hirohisa
中科院分区：
文献类型：
--
作者：
Naota, Misaki;Shiotsu, Shizuka;Takano, Hirohisa
亚洲沙尘（ASD）事件与肺部发病率和死亡率的增加有关。自2000年以来，东亚地区ASD事件的数量迅速增加。为了研究ASD的慢性肺毒性，将低剂量（200和400 μ g）和高剂量（800和3，000 μ g）ASD的盐水悬浮液经气管内滴注到ICR小鼠中。在滴注后24小时、1周或1、2或3个月处死动物。组织学检查显示ASD在滴注后24小时引起急性炎症。急性炎症为一过性，滴注后1周和1个月消退。在滴注后2个月和3个月，偶尔观察到淋巴细胞局灶性浸润伴上皮样巨噬细胞蓄积（提示转化为肉芽肿）和肉芽肿形成。在高剂量组中，滴注后3个月在肺淋巴结中观察到含颗粒的巨噬细胞聚集。长期的炎性病灶（肉芽肿）和肺淋巴结中ASD颗粒的存在有可能诱导免疫调节，导致暴露动物的不良健康影响。
Asian sand dust (ASD) events are associated with an increase in pulmonary morbidity and mortality. The number of ASD events has increased rapidly in the east Asian region since 2000. To study the chronic lung toxicity of ASD, saline suspensions of low doses (200 and 400 mu g) and high doses (800 and 3,000 mu g) of ASD were intratracheally instilled into ICR mice. Animals were sacrificed at 24 hr, 1 week, or 1, 2, or 3 months after instillation. Histopathological examination revealed that ASD induced acute inflammation at 24 hr after instillation. The acute inflammation was transient and subsided at 1 week and 1 month after instillation. At 2 and 3 months after instillation, focal infiltration of lymphocytes with accumulation of epithelioid macrophages, which is a suggestive finding of transformation to granuloma, and granuloma formation were occasionally observed. Aggregation of macrophages containing particles was observed in the pulmonary lymph nodes at 3 months after instillation in high-dose groups. Prolonged inflammatory foci (granuloma) and presence of ASD particles in pulmonary lymph nodes would have a chance to induce immunological modulation leading to adverse health effects in the exposed animals.