Biosynthesis of gangliosides from asialogangliosides in rat liver Golgi vesicles.
Biosynthesis of gangliosides from asialogangliosides in rat liver Golgi vesicles.
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大鼠肝高尔基体小泡中去唾液酸神经节苷脂的生物合成。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
K. Sandhoff
中科院分区：
文献类型：
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作者：
G. Pohlentz;D. Klein;D. Schmitz;G. Schwarzmann;J. Peter;K. Sandhoff
研究了从大鼠肝脏分离的高尔基囊泡中糖脂GA2、GA1、GM1b和GD1c的生物合成。将n -乙酰半乳糖胺、半乳糖和两种唾液酸残基依次加入到乳糖神经酰胺中得到最终产物GD1c。相应的糖基转移酶的活性被证明存在于分离的高尔基囊泡中，并确定了它们各自的动力学数据。每个反应的产物在薄层色谱上的迁移率，酶降解到各自的前体，以及在GM1b的情况下用FAB质谱法表征。
Biosynthesis of glycolipids GA2, GA1, GM1b, and GD1c was studied in Golgi vesicles isolated from rat liver. Sequential addition of N-acetylgalactosamine, galactose and two sialic acid residues to lactosylceramide led to the endproduct GD1c. Activities of the corresponding glycosyltransferases were shown to be present in isolated Golgi vesicles and their respective kinetic data were determined. The products of each reaction were characterized by their mobility on thin-layer chromatography, by enzymic degradation to their respective precursors, and in case of GM1b by FAB mass spectrometry.