Deciphering source and sink responses of potato plants (Solanum tuberosum L.) to elevated temperatures
Deciphering source and sink responses of potato plants (Solanum tuberosum L.) to elevated temperatures
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DOI：
10.1111/pce.13366
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发表时间：
2018-11-01
影响因子：
7.3
通讯作者：
Sonnewald, Sophia
中科院分区：
文献类型：
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作者：
Hastilestari, Bernadetta Rina;Lorenz, Julia;Sonnewald, Sophia
马铃薯是一种重要的主食，在世界范围内越来越受欢迎。温度升高显著损害块茎产量和质量。因此，培育耐热品种是确保未来马铃薯可持续生产的迫切需要。综合的方法相结合的生理学，生物化学和分子生物学进行，以有助于更好地了解热对源（叶）和库器官（块茎）在热敏感品种的影响。设计了允许组织特定热应用的实验装置。昼夜温度升高（29摄氏度/27摄氏度）会损害光合作用和同化物的生产。生物量分配转向远离块茎对叶表明发展中块茎的库强度降低。降低库强度的块茎是通过降低蔗糖合成酶活性和表达在高温下。热介导的块茎生长抑制与韧皮部移动块茎化信号SP 6A在叶片中的表达减少相一致。SP 6A的表达和光合作用也受到影响，当只有地下空间被加热，叶片保持在控制条件下。相反，块茎代谢的负面影响减弱，当只有拍摄受到高温。这一点，连同转录变化的讨论，表明叶和块茎之间的双向通信，以调整源容量和/或库强度的环境条件。
Potato is an important staple food with increasing popularity worldwide. Elevated temperatures significantly impair tuber yield and quality. Breeding heat-tolerant cultivars is therefore an urgent need to ensure sustainable potato production in the future. An integrated approach combining physiology, biochemistry, and molecular biology was undertaken to contribute to a better understanding of heat effects on source- (leaves) and sink-organs (tubers) in a heat-susceptible cultivar. An experimental set-up was designed allowing tissue-specific heat application. Elevated day and night (29 degrees C/27 degrees C) temperatures impaired photosynthesis and assimilate production. Biomass allocation shifted away from tubers towards leaves indicating reduced sink strength of developing tubers. Reduced sink strength of tubers was paralleled by decreased sucrose synthase activity and expression under elevated temperatures. Heat-mediated inhibition of tuber growth coincided with a decreased expression of the phloem-mobile tuberization signal SP6A in leaves. SP6A expression and photosynthesis were also affected, when only the belowground space was heated, and leaves were kept under control conditions. By contrast, the negative effects on tuber metabolism were attenuated, when only the shoot was subjected to elevated temperatures. This, together with transcriptional changes discussed, indicated a bidirectional communication between leaves and tubers to adjust the source capacity and/or sink strength to environmental conditions.