Invasive Potential of Gut Mucosa-derived Fusobacterium nucleatum Positively Correlates with IBD Status of the Host
Invasive Potential of Gut Mucosa-derived Fusobacterium nucleatum Positively Correlates with IBD Status of the Host
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DOI：
10.1002/ibd.21606
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发表时间：
2011-09-01
影响因子：
4.9
通讯作者：
Allen-Vercoe, Emma
中科院分区：
文献类型：
--
作者：
Strauss, Jaclyn;Kaplan, Gilaad G.;Allen-Vercoe, Emma
背景：具核梭杆菌是一种异质口腔病原体，也是人类肠道粘膜的常见居民。鉴于已知一些具核梭杆菌菌株在口腔粘膜中具有侵袭性和促炎性，我们将从炎症性肠病 (IBD) 患者中分离的菌株与从健康对照中分离的菌株进行比较，以确定 I) 该物种是否更常见于 IBD 患者； 2) 来自 IBD 患者的肠道来源的具核梭杆菌菌株是否表现出增强的侵袭能力。 方法：从 56 名接受结肠镜检查的成人患者中获取活检材料，用于结肠癌筛查或评估肠易激综合征状态（34 名患者），或评估是否存在胃肠道疾病（即 IBD 或不确定性结肠炎，22 名患者）。我们列举了梭杆菌属。以盲法方式从人类肠道活检材料中分离出菌株，然后在 Caco-2 模型系统中使用入侵测定来比较部分 F. micleatum 菌株的毒力潜力。 结果：梭杆菌属。从 63.6% 的胃肠道疾病患者中分离出这种细菌，而在健康对照中这一比例为 26.5%（P = 0.01）。总共有 69% 的梭杆菌属。从患者体内回收的细菌被鉴定为具核梭菌。在 Caco-2 细胞侵袭测定中，源自 IBD 患者发炎活检组织的 F. 成核菌株比从 IBD 患者或对照患者的健康组织中分离的菌株更具侵袭性（P < 0.05 至 0.001）。 结论：本研究表明，高度侵袭性的 F. 成核菌株在肠粘膜上的定植可能是 F. 成核菌株的有用生物标志物。 胃肠道疾病。
Background: Fusobacterium nucleatum is a heterogeneous oral pathogen that is also a common resident of the human gut mucosa. Given that some strains of F. nucleatum are known to be invasive and proinflammatory in the oral mucosa, we compared strains isolated from patients with inflammatory bowel disease (IBD) with strains isolated from healthy controls to determine I) whether this species was more commonly associated with IBD patients; and 2) whether gut-derived F. nucleatum strains from IBD patients showed an increased capacity for invasion.Methods: Biopsy material was obtained from 56 adult patients undergoing colonoscopy for colon cancer screening purposes or assessment of irritable bowel syndrome status (34 patients), or to assess for presence of gastrointestinal disease (i.e.. IBD or indeterminate colitis, 22 patients). We enumerated Fusobacterium spp. strains isolated from human gut biopsy material in a blinded fashion, and then compared the virulence potential of a subset of F. micleatum strains using an invasion assay in a Caco-2 model system.Results: Fusobacterium spp. were isolated from 63.6% of patients with gastrointestinal disease compared to 26.5% of healthy controls (P = 0.01). In total, 69% of all Fusobacterium spp. recovered from patients were identified as F. nucleatum. F. nucleation strains originating from inflamed biopsy tissue from IBD patients were significantly more invasive in a Caco-2 cell invasion assay than strains that were isolated from healthy tissue from either IBD patients or control patients (P < 0.05 to 0.001).Conclusions: This study indicates that colonization of the intestinal mucosa by highly invasive strains of F. nucleation may be a useful biomarker for gastrointestinal disease.