Changes in endophytic bacterial communities during different growth stages of cucumber (Cucumis sativus L.)
Changes in endophytic bacterial communities during different growth stages of cucumber (Cucumis sativus L.)
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DOI：
10.1007/s11274-019-2676-z
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发表时间：
2019-07-01
影响因子：
4.1
通讯作者：
Kataoka, Ryota
中科院分区：
文献类型：
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作者：
Mahmood, Ahmad;Takagi, Kazuhiro;Kataoka, Ryota
植物中的内生细菌多样性代表了可培养和不可培养内生细菌之间的相互作用水平，从而提供了对内生环境的适当洞察。这项研究是为了确定可培养和非可培养内生细菌在包括四个连续生长阶段的两个不同地点的趋势。对于可培养内生细菌，采用稀释板技术进行分离，获得的菌落进行聚合酶链式反应-限制性片段长度多态性(RFLP)比较。通过16S rRNA测序，将不同的RFLP类型与其最近的近邻进行了鉴定。通过下一代测序获得非可培养内生细菌多样性。结果表明，在观察到的可操作分类单位和多样性指数方面，可培养细菌和非可培养细菌之间的趋势相似。值得注意的是，阶段1中的内生细菌消失了，取而代之的是不同的内生细菌出现。此外，还观察到某些可培养细菌和非可培养细菌的时间持久性。综上所述，黄瓜内生细菌多样性随着植株的生长发育而先增加后降低。此外，有人认为，植物在整个生命周期中调节内生菌的数量和多样性。
Endophytic bacterial diversity in plants presents the level of interaction between culturable and non-culturable endophytic bacteria, thereby providing an appropriate insight into the endophytic environment. This study was conducted to determine the trend of culturable and non-culturable endophytic bacteria at two different sites encompassing four consecutive growth stages. For culturable endophytic bacteria, isolation was carried out using the dilution plate technique, and the obtained colonies were compared using PCR-restriction fragment length polymorphism (RFLP). Different RFLP-types were identified to their nearest neighbour using 16S rRNA sequencing. The non-culturable endophytic bacterial diversity was obtained by next generation sequencing. Results suggested a similar trend among the culturable and non-culturable bacteria for observed operational taxonomic units and diversity indices. It is noticeable that the endophytic bacteria inhabiting in stage 1 disappeared, and instead, different endophytic bacteria appeared. Moreover, the temporal persistence of certain culturable and non-culturable bacteria was also observed. In conclusion, the endophytic bacterial diversity in cucumber initially increased with the plant growth and then decreased at a later stage. Furthermore, it was suggested that plants regulate the number and diversity of endophytes throughout the lifecycle of plants.