Distinct Patterns of PPAR Promoter Usage, Lipid Degradation Activity, and Gene Expression in Subcutaneous Adipose Tissue of Lean and Obese Swine
Distinct Patterns of PPAR Promoter Usage, Lipid Degradation Activity, and Gene Expression in Subcutaneous Adipose Tissue of Lean and Obese Swine
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瘦猪和肥胖猪皮下脂肪组织中 PPARγ 启动子使用、脂质降解活性和基因表达的不同模式
DOI：
10.3390/ijms19123892
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发表时间：
2018-12-01
影响因子：
5.6
通讯作者：
Wang, Xibiao
中科院分区：
文献类型：
--
作者：
Song, Bin;Di, Shengwei;Wang, Xibiao
皮下脂肪组织是一种疏松的结缔组织，专门调节能量储存和代谢。在家猪（Sus scrofa）中，皮下脂肪组织的时间发育对于产肉至关重要。然而，脂肪组织发育的调节仍不清楚。在此，在幼龄和幼龄至成年生长阶段，对瘦肉猪（Danish-Landrace）和肥胖猪（Min）的皮下脂肪组织发育进行了表征和比较。使用RNA测序，我们分析了从4周龄和16周龄猪分离的皮下脂肪组织的转录组，并鉴定了24，718个表达的转录单位。其中，6327个基因的品种和/或发育阶段之间的差异表达。与肥胖猪相比，瘦肉型猪中上调基因在脂肪酸降解和线粒体功能方面表现出显著的功能和途径富集。进一步的分析揭示了与两个品种皮下脂肪组织发育相关的三种替代过氧化物酶体增殖物激活受体γ（PPARγ）启动子的不同使用偏好。对瘦肉型猪和肥胖型猪皮下脂肪组织的转录组分析表明，脂肪酸降解和PPARγ信号通路的标记辅助选择可能成为未来猪肉品质改良和现代育种的重要方向。
Subcutaneous adipose tissue is a loose connective tissue specializing in the regulation of energy storage and metabolization. In domesticated pigs (Sus scrofa), the temporal development of subcutaneous adipose tissue is critical for meat production. However, the regulation of adipose tissue development remains unclear. Here, the subcutaneous adipose tissue development was characterized and compared in lean (Danish-Landrace) and obese (Min) pigs at juvenile and the juvenile-to-adult growth stages. Using RNA sequencing, we profiled the transcriptome of subcutaneous adipose tissue isolated from 4- and 16-week-old pigs and identified 24,718 expressed transcription units. Of them, 6327 genes were differentially expressed between the breeds and/or developmental stages. Compared with obese pigs, upregulated genes in lean pigs showed significant function and pathway enrichment in fatty acid degradation and mitochondrial functions. Further analysis uncovered the distinct usage preferences of the three alternative peroxisome proliferator-activated receptor γ (PPARγ) promoters associated with the development of subcutaneous adipose tissue in both breeds. Transcriptome analysis of subcutaneous adipose tissue in lean and obese pigs suggested that marker-assisted selection of fatty acid degradation and PPARγ signaling pathways could be important directions for future pork quality improvement and modern breeding.