The effects of modified sini decoction on liver injury and regeneration in acute liver failure induced by D-galactosamine in rats
The effects of modified sini decoction on liver injury and regeneration in acute liver failure induced by D-galactosamine in rats
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DOI：
10.1016/j.jep.2014.12.003
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发表时间：
2015-02-23
影响因子：
5.4
通讯作者：
Zhu, Hong
中科院分区：
文献类型：
--
作者：
Luo, Jianxing;Zhang, Yang;Zhu, Hong
民族药理相关性：加味四逆汤(MSND)是一种著名的中医方剂，多年来一直用于治疗心血管和肝脏疾病。材料与方法：用D-氨基半乳糖(D-GalN)诱导雄性Wistar大鼠急性肝功能衰竭。接下来，对大鼠灌胃给予更强的Neo-Minophagen C和MSND。观察生化指标、肝脏组织学变化、大鼠存活情况及Toll样受体4(TLR4)、核因子-kappaB(NF-kappa B)、高迁移率族蛋白1(HMGB1)、半胱氨酸天冬氨酸氨基转移酶-3(caspase-3)和增殖细胞核抗原(PC NA)的表达。较强的SNMC和MSND均能改善小鼠的生化指标，减少肝组织的坏死，但MSND的作用更强。SNMC和MSND均能显著降低HMGB1、TLR4、NF-kappaB、Caspase-3mRNA的表达，上调增殖细胞核抗原的表达，且MSND的作用更强。结论：MSND对急性肝功能衰竭大鼠有保护作用，提高了存活率。这些作用可能是通过抑制炎症反应和细胞凋亡，促进肝组织再生而实现的。(C)2014爱思唯尔爱尔兰有限公司。保留所有权利。
Ethnopharmacological relevance: Modified sini decoction (MSND) is a well-known traditional Chinese medical formula that has been used to treat cardiovascular and liver diseases for many years. We investigated the effects of MSND on acute liver failure and identified the possible mechanisms of these effects.Material and methods: Acute liver failure was induced by intraperitoneal injection of D-galactosamine (D-GalN) into specific pathogen-free male Wistar rats. Next, the rats were treated with Stronger Neo-Minophagen C and MSND via gavage. Biochemical parameters, histological changes in the liver, the survival of rats and the mRNA levels of toll-like receptor 4 (TLR4), nuclear factor kappa B (NF-kappa B), high mobility group box 1 (HMGB1) caspase-3 and proliferating cell nuclear antigen (PCNA) were analyzed.Results: MSND prolonged the survival times of the acute liver failure rats. The biochemical parameters were improved, and necrosis in the liver tissues was reduced by both Stronger Neo-Minophagen C (SNMC) and MSND, but MSND induced greater effects. The mRNA expressions of HMGB1, TLR4, NF-kappa B, and Caspase-3 were remarkably decreased, and the expression of PCNA was remarkably increased by SNMC and MSND, and the effects of MSND were greater.Conclusions: MSND protected the liver and increased the survival rate of acute liver failure rats. These effects were likely mediated by the inhibitions of the inflammatory reaction and apoptosis and the promotion of liver tissue regeneration. (C) 2014 Elsevier Ireland Ltd. All rights reserved.