Monitoring voltage fluctuations of intracellular membranes
Monitoring voltage fluctuations of intracellular membranes
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DOI：
10.1038/s41598-018-25083-7
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发表时间：
2018-05-02
影响因子：
4.6
通讯作者：
Baker, Bradley J.
中科院分区：
文献类型：
--
作者：
Rad, Masoud Sepehri;Cohen, Lawrence B.;Baker, Bradley J.
在真核细胞中，内质网（ER）是最大的连续的膜封闭的网络，它包围着一个单一的腔。利用一种新的基因编码电压指示器（GEVI），我们将膜片钳技术应用于培养的HEK 293细胞和神经元，发现质膜和内膜之间存在非常快的电相互作用。这一发现表明了外膜和内膜之间相互作用的新机制以及各种内膜之间相互作用的机制。ER可以在质膜和其他内部细胞器之间传递电信号。内膜光信号极性相反，但具有与质膜信号相似的时间过程。质膜中GEVI的光学信号在试验之间是一致的。然而，内部信号的大小随着重复试验而减小，表明电耦合正在退化和/或内膜的电阻正在衰减。
In eukaryotic cells, the endoplasmic reticulum (ER) is the largest continuous membrane-enclosed network which surrounds a single lumen. Using a new genetically encoded voltage indicator (GEVI), we applied the patch clamp technique to cultured HEK293 cells and neurons and found that there is a very fast electrical interaction between the plasma membrane and internal membrane(s). This discovery suggests a novel mechanism for interaction between the external membrane and internal membranes as well as mechanisms for interactions between the various internal membranes. The ER may transfer electrical signals between the plasma membrane and other internal organelles. The internal membrane optical signal is reversed in polarity but has a time course similar to that of the plasma membrane signal. The optical signal of the GEVI in the plasma membrane is consistent from trial to trial. However, the internal signal decreases in size with repeated trials suggesting that the electrical coupling is degrading and/or the resistance of the internal membrane is decaying.