Differential contributions of connexin37 and connexin43 to oogenesis revealed in chimeric reaggregated mouse ovaries
Differential contributions of connexin37 and connexin43 to oogenesis revealed in chimeric reaggregated mouse ovaries
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DOI：
10.1242/jcs.02624
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发表时间：
2005-11-01
影响因子：
4
通讯作者：
Kidder, GM
中科院分区：
文献类型：
--
作者：
Gittens, JEI;Kidder, GM
小鼠卵巢卵泡形成需要缝隙连接蛋白connexin37和connexin43。为了确定它们各自在卵子发生中的作用，将含有零突变卵母细胞和野生型颗粒细胞或相反组合的嵌合卵巢移植到免疫缺陷雌性小鼠的肾囊中。三周后，对卵母细胞进行体外减数分裂能力和受精率测试。由缺乏connexin43的卵母细胞和野生型颗粒细胞组成的卵巢产生包围卵母细胞的窦卵泡，这些卵母细胞至少可以发育到双细胞阶段，这表明卵母细胞不需要表达connexin43来达到成熟。相反，在含有野生型卵母细胞和缺乏connexin43的颗粒细胞或缺乏connexin37的卵母细胞和野生型颗粒细胞的卵巢中，卵泡发育和卵母细胞成熟都受到损害。因此，颗粒细胞中连接蛋白43或卵母细胞中连接蛋白37的缺失足以损害卵母细胞和卵泡的发育。野生型卵母细胞与缺乏连接蛋白37的颗粒细胞配对产生含有卵母细胞的窦卵泡，卵母细胞至少发育到双细胞阶段。因此，颗粒细胞中连接蛋白37的缺失并不会影响小鼠的生育能力。染料转移实验显示卵母细胞-颗粒细胞偶联在这些卵泡中持续存在，表明另一种连接蛋白的功能补偿。结果表明，小鼠卵母细胞不需要表达connexin43来发育成减数分裂能力强、可受精的配子，但必须表达connexin37来与颗粒细胞交流，这是卵子发生的必要条件。
The gap junction proteins connexin37 and connexin43 are required for ovarian folliculogenesis in the mouse. To define their respective roles in oogenesis, chimeric ovaries containing either null mutant oocytes and wild-type granulosa cells or the reverse combination were grafted to the renal capsules of immunodeficient female mice. After three weeks, the oocytes were tested for meiotic competence and fertilizability in vitro. Ovaries composed of connexin43-deficient oocytes and wild-type granulosa cells produced antral follicles enclosing oocytes that could develop to at least the two-cell stage, demonstrating that oocytes need not express connexin43 to reach maturity. Conversely, both follicle development and oocyte maturation were impaired in ovaries containing either wild-type oocytes and connexin43-deficient granulosa cells or connexin37-deficient oocytes and wild-type granulosa cells. Thus absence of connexin43 from granulosa cells or connexin37 from oocytes is sufficient to compromise both oocyte and follicle development. Wild-type oocytes paired with connexin37-deficient granulosa cells generated antral follicles containing oocytes that developed to at least the two-cell stage. Therefore, connexin37 absence from granulosa cells need not impair fertility in mice. Dye transfer experiments revealed persistent oocyte-granulosa cell coupling in those follicles, indicating functional compensation by another connexin. The results indicate that mouse oocytes do not need to express connexin43 in order to develop into meiotically competent, fertilizable gametes, but must express connexin37 for communication with granulosa cells, a requirement for oogenesis.