The genetic basis of sex determination in grapes
The genetic basis of sex determination in grapes
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DOI：
10.1038/s41467-020-16700-z
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发表时间：
2020-06-09
影响因子：
16.6
通讯作者：
Cantu, Dario
中科院分区：
文献类型：
--
作者：
Massonnet, Melanie;Cochetel, Noe;Cantu, Dario
鉴定导致植物性别分化的基因和突变仍然是一个重大挑战。本文研究了葡萄属植物性别决定区（SDR）的结构和进化。我们报告了一个改进的，染色体规模的赤霞珠基因组序列和9个野生和栽培葡萄基因组的分阶段组装。通过解析20个葡萄属SDR单倍型，我们比较了雄性、雌性和雌雄同体单倍型结构并鉴定了性连锁区域。再加上基因表达数据，我们确定了一个候选的雄性不育突变VviINP1基因和潜在的女性不育功能与转录因子VviYABBY3。我们的数据表明，在驯化过程中，通过雄性和雌性单倍型之间的罕见重组事件，diodectin已经丢失。这项工作显着推进了对葡萄属性别决定遗传基础的了解，并为葡萄育种计划中快速识别性别类型提供了必要的信息。葡萄藤是少数祖先雌雄异株的作物之一，在驯化过程中恢复为雌雄同体。在这里，作者确定了与葡萄雄性和雌性不育相关的候选基因，并描述了在驯化过程中导致两性畸形的遗传过程。
It remains a major challenge to identify the genes and mutations that lead to plant sexual differentiation. Here, we study the structure and evolution of the sex-determining region (SDR) in Vitis species. We report an improved, chromosome-scale Cabernet Sauvignon genome sequence and the phased assembly of nine wild and cultivated grape genomes. By resolving twenty Vitis SDR haplotypes, we compare male, female, and hermaphrodite haplotype structures and identify sex-linked regions. Coupled with gene expression data, we identify a candidate male-sterility mutation in the VviINP1 gene and potential female-sterility function associated with the transcription factor VviYABBY3. Our data suggest that dioecy has been lost during domestication through a rare recombination event between male and female haplotypes. This work significantly advances the understanding of the genetic basis of sex determination in Vitis and provides the information necessary to rapidly identify sex types in grape breeding programs. Grapevine is one of a few ancestrally dioecious crops that are reverted to hermaphroditism during domestication. Here, the authors identify candidate genes related to male- and female-sterility in grapes and describe the genetic process that led to hermaphroditism during domestication.