Oligo( l -glutamic acids) in Calcium Phosphate Precipitation: Chain Length Effect
Oligo( l -glutamic acids) in Calcium Phosphate Precipitation: Chain Length Effect
复制标题
磷酸钙沉淀中的低聚（L-谷氨酸）：链长效应
DOI：
10.1021/acs.jpcb.0c01689
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Sahai, Nita
中科院分区：
文献类型：
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作者：
Ustriyana, Putu;Harmon, Emma;Chen, Kexun;Michel, F. Marc;Sahai, Nita
对磷酸钙沉淀的理解是不同科学领域的主要兴趣，包括医学，生物材料和物理化学。已知添加剂生物大分子的存在会影响从成核到晶体生长的沉淀过程的各个阶段。在目前的工作中，受天然生物矿化蛋白中连续的酸性氨基酸序列的启发，利用定义明确的短序列，带负电荷的肽，寡聚（l-谷氨酸）作为模型。通过分析沉淀物的形态、物相、元素在溶液和沉淀物中的时间分布以及沉淀过程的动力学来解释这些短肽对磷酸钙沉淀的影响。结果表明，多肽可以延缓无定形前体相向羟基磷灰石的相变，并且在一定浓度的多肽下存在最佳链长。本研究是两部分系列的第一部分，随后的工作将揭示这些短肽影响磷酸钙沉淀的机制。
The understanding of calcium phosphate precipitation is of major interest in different fields of science, including medicine, biomaterials, and physical chemistry. The presence of additive biomacromolecules has been known to influence various stages of the precipitation process from nucleation to crystal growth. In the current work, well-defined sequences of short, negatively charged peptides, oligo(l-glutamic acids), were utilized as a model, inspired by contiguous sequences of acidic amino acids in natural biomineralization proteins. The precipitate morphology and phases, the element time profile in solution and in the precipitates, as well as the kinetics during the precipitation process were analyzed to explain the effect of these short peptides on calcium phosphate precipitation. The results show that peptides can delay the phase transformation of an amorphous precursor phase to hydroxyapatite and that there is an optimal chain length for this effect at a given concentration of peptide. This study is the first part of a two-part series and is followed by a subsequent work to reveal the mechanism by which these short peptides influence the calcium phosphate precipitation.