Filtration characteristics of the single isolated perfused glomerulus of Myxine glutinosa.
Filtration characteristics of the single isolated perfused glomerulus of Myxine glutinosa.
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Myxine glutinosa 单个分离的灌注肾小球的过滤特性。
DOI：
10.1159/000173773
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Stolte,H
中科院分区：
文献类型：
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作者：
Fels,LM;Sanz-Altamira,PM;Decker,B;Elger,B;Stolte,H
采用体外微灌注技术研究了大西洋盲鳗（Myxine glutinosa）单个离体肾小球的滤过特性。评价了该方法作为研究肾小球功能模型的适用性。无蛋白灌注液的过滤系数Kf（0.189±0.181 nl × s-1× mmHg-1）与其他脊椎动物体外测定的过滤系数相同。连续薄片（1µm）形态计量学分析显示肾小球毛细血管表面(a)为1.84±0.72 mm2。肾小球直径与A呈线性关系，可以计算出肾小球的水导率Lp（0.618±0.384µl × s-1× mmHg-1× cm-2）。结果表明，m。谷氨酸盐具有与高等脊椎动物相似的过滤特性。m。与其他动物的肾小球相比，谷氨酰胺易于解剖，是研究脊椎动物肾小球滤过性和渗透性特征的合适模型。该模型可能应用的一个例子是研究肾上腺素对肾小球滤过特性的影响。肾上腺素导致大鼠超滤压（PUF）、肾小球滤过率（SNGFR）和滤过分数（FF）升高；Kfremained未受影响。
Using an in vitro microperfusion technique the filtration characteristics of single isolated glomeruli of the Atlantic hagfish(Myxine glutinosa)were investigated. The suitability of the method as a model to study glomerular function was evaluated. Experiments with a protein-free perfusate led to a filtration coefficient Kf(0.189 ± 0.181 nl × s-1× mmHg-1) that was in the same order of magnitude as determined in vitro for other vertebrates. Morphometric analysis on serial thin sections (1 µm) revealed a glomerular capillary surface (A) of 1.84 ± 0.72 mm2. A linear relationship between glomerular diameter and A allowed the calculation of the hydraulic conductivity Lp(0.618 ± 0.384 µl × s-1× mmHg-1× cm-2). The data indicate that the isolated perfused glomerulus ofM. glutinosahas filtration characteristics similar to higher vertebrates. The glomeruli ofM. glutinosa,which in contrast to glomeruli of other animals are easy to dissect, are a suitable model for the study of glomerular filtration and permeability characteristics of vertebrates. An example of a possible application of the model is a study into the effects of adrenaline on glomerular filtration characteristics. Adrenaline led to increases in ultrafiltration pressure (PUF) > single nephron glomerular filtration rate (SNGFR) and filtration fraction (FF); Kfremained unaffected.