Luminescence due to peptide linkage observed in L-cysteine molecules irradiated by infrared laser light
Luminescence due to peptide linkage observed in L-cysteine molecules irradiated by infrared laser light
复制标题
DOI：
10.1016/j.jlumin.2015.08.070
复制
发表时间：
2016
影响因子：
3.6
通讯作者：
T. Tsujibayashi;E. Matsubara;M. Ichimiya;N. Ohno
中科院分区：
文献类型：
--
作者：
T. Tsujibayashi;E. Matsubara;M. Ichimiya;N. Ohno
由蛋白质组成的肽链中氨基酸的序列是生物最基本的信息。迫切需要一种直接的、无损的读取方法来替代基于基因分析的方法。发光探测是一种非常灵敏的探测各种材料的工具。为了找到每一种氨基酸的特征发光，我们使用脉冲持续时间为1.5 ps的3.36 eV紫外激光对l -半胱氨酸和l -酪氨酸进行了研究。除了常见的2.66 eV发光带外，我们还发现l -半胱氨酸和l -酪氨酸的发光带分别为2.89 eV和2.92 eV。可以解释为侧链通过影响肽链或羰基对发光产生影响。
The sequence of amino acids in peptide chains consisting of proteins is the most fundamental information of living things. A direct and nondestructive method of reading is highly required as an alternative to the method based on the gene analysis. Luminescence detection is a very sensitive tool for investigating various materials. In order to find characteristic luminescence of each amino acid we study L-cysteine and L-tyrosine using UV laser of 3.36 eV with pulse duration of 1.5 ps. In addition to a common 2.66 eV band of the luminescence we have found 2.89 eV band for L-cysteine and 2.92 eV band for L-tyrosine. It can be interpreted that the side chain makes difference on the luminescence by affecting the peptide linkage or carbonyl group.