Melanoma-associated antigens as messenger RNA detection markers for melanoma.
Melanoma-associated antigens as messenger RNA detection markers for melanoma.
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DOI：
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发表时间：
1997-04
期刊：
影响因子：
11.2
通讯作者：
T. Sarantou;D. Chi;D. Garrison;A. Conrad;P. Schmid;D. Morton;D. Hoon
中科院分区：
文献类型：
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作者：
T. Sarantou;D. Chi;D. Garrison;A. Conrad;P. Schmid;D. Morton;D. Hoon
黑色素瘤的生物学特性和黑色素瘤相关抗原（MAAs）是异质性的。这种多样性在参与黑色素合成途径的MAA的表达中部分观察到。因此，我们开发了一种敏感的多标记逆转录-PCR加Southern印迹法，使用5个MAA作为分子标记，以检测原发性和转移性黑色素瘤细胞。评估黑色素瘤细胞系、黑色素细胞（培养的）、原发性和转移性恶性黑色素瘤组织以及来自美国癌症联合委员会I-IV期黑色素瘤患者的血液的酪氨酸酶、酪氨酸酶相关蛋白1和2、Pmel 17和MART-1/Melan-A。所有MAA mRNA标记物在100%的黑色素瘤细胞系和培养的黑色素细胞、74%的原发性和转移性肿瘤（不包括肿瘤引流淋巴结）、43%的肿瘤累及淋巴结和43%的患者血液中表达。低黑素性黑色素瘤组织表达单个mRNA标记物的频率较低。总体而言，至少一种mRNA标记物在超过86%的分析标本中表达。来自患者的正常组织标本和来自正常志愿者供体的血液对MAA mRNA表达呈阴性。多标记MAA逆转录-PCR加Southern印迹分析比单分子标记检测黑色素瘤细胞更可靠和敏感。该分子测定还可以提供关于转移性黑素瘤细胞的MAA mRNA表达的信息，其可以帮助监测针对携带特定MAA的黑素瘤的主动特异性免疫疗法的治疗功效。
Melanoma is heterogeneous for its biological properties and melanoma-associated antigens (MAAs). This diversity is partially observed in the expression of the MAAs involved with the melanin synthesis pathway. We therefore developed a sensitive multimarker reverse transcription-PCR plus Southern blot assay using five MAAs as molecular markers to detect primary and metastatic melanoma cells. Melanoma cell lines, melanocytes (cultured), primary and metastatic malignant melanoma tissues, and blood from patients with American Joint Committee on Cancer stage I-IV melanoma were assessed for tyrosinase, tyrosinase-related proteins 1 and 2, Pmel 17, and MART-1/Melan-A. All of the MAA mRNA markers were expressed in 100% of melanoma cell lines and cultured melanocytes, 74% of primary and metastatic tumors (excluding tumor-draining lymph nodes), 43% of tumor-involved lymph nodes, and 43% of patients' bloods. Hypomelanotic melanoma tissues expressed a lower frequency of individual mRNA markers. Overall, at least one mRNA marker was expressed in more than 86% of specimens assayed. Normal tissue specimens from patients and blood from normal volunteer donors were negative for MAA mRNA expression. The multimarker MAA reverse transcription-PCR plus Southern blot analysis was more reliable and sensitive than a single-molecular marker assay for the detection of melanoma cells. This molecular assay can also provide information on MAA mRNA expression of metastatic melanoma cells that may assist in monitoring the therapeutic efficacy of active specific immunotherapy toward specific MAA-bearing melanomas.