Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride.
Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride.
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DOI：
10.1073/pnas.79.16.4888
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发表时间：
1982-08
影响因子：
11.1
通讯作者：
P. Sternweis;A. Gilman
中科院分区：
文献类型：
--
作者：
P. Sternweis;A. Gilman
F-激活纯化的腺苷环化酶鸟嘌呤核苷酸结合调节成分(G/F)需要镁离子和另一种因素的存在。这种污染各种核苷酸商业制剂和一次性玻璃试管的因素已被确定为Al3+。在10 mM镁离子和5 mM F-存在下，AlCl3引起G/F的活化，其表观活化常数约为1-5微米。对Al3+的要求是非常特殊的；在其他28种被测试的金属中，只有Be2+促进了F-对G/F的激活。
Activation of the purified guanine nucleotide-binding regulatory component (G/F) of adenylate cyclase by F- requires the presence of Mg2+ and another factor. This factor, which contaminates commercial preparations of various nucleotides and disposable glass test tubes, has been identified as Al3+. In the presence of 10 mM Mg2+ and 5 mM F-, AlCl3 causes activation of G/F with an apparent activation constant of approximately 1-5 muM. The requirement for Al3+ is highly specific; of 28 other metals tested, only Be2+ promoted activation of G/F by F-.