Translational control in sea urchin eggs and embryos: initiation is rate limiting in blastula stage embryos.
Translational control in sea urchin eggs and embryos: initiation is rate limiting in blastula stage embryos.
复制标题
海胆卵和胚胎的翻译控制：囊胚阶段胚胎的起始是速率限制的。
DOI：
10.1016/0012-1606(81)90336-5
复制
发表时间：
1981
影响因子：
2.7
通讯作者：
Moon,RT
中科院分区：
文献类型：
--
作者：
Hille,MB;Hall,DC;Yablonka-Reuveni,Z;Danilchik,MV;Moon,RT
为了确定在海胆胚胎发生过程中，起始是否限制了蛋白质合成的速率，在存在和不存在少量多肽延伸抑制剂emetine的情况下，对未受精卵和卵裂期、囊胚期和棱镜期胚胎的多核糖体谱进行了检测。在卵形成过程中，通过注射[3H]尿苷对核糖体进行放射性标记，以便监测单核糖体向多核糖体的转移。虽然在未受精卵或2- 16细胞的紫圆梭胚胎中，起始率不受限制，但在囊胚和棱柱胚中，起始率是受限制的。我们认为起始成为速率限制，以允许在后期发育中选择性翻译某些类别的mRNA。
To determine whether initiation is rate-limiting in protein synthesis during the embryogenesis of sea urchins, polyribosome profiles of unfertilized eggs and cleavage, blastula and prism stage embryos were examined after incubation of the eggs and embryos in the presence and absence of low amounts of emetine, an inhibitor of polypeptide elongation. The ribosomes were radioactively labeled with [3H]uridine by injection of the adults during oogenesis so that we could monitor emetine-dependent shifts of monoribosomes to polyribosomes. Although initiation is not rate limiting in unfertilized eggs or 2- to 16-cell embryos of Strongylocentrotus purpuratus, it is rate limiting in blastula and prism embryos. We suggest that initiation becomes rate limiting to allow the selective translation of certain classes of mRNA during later development.