Effects of four monoterpenes on germination, primary root growth, and mitochondrial respiration of maize
Effects of four monoterpenes on germination, primary root growth, and mitochondrial respiration of maize
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DOI：
10.1023/a:1005467903297
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发表时间：
2000-03-01
影响因子：
2.3
通讯作者：
Ishii-Iwamoto, EL
中科院分区：
文献类型：
--
作者：
Abrahim, D;Braguini, WL;Ishii-Iwamoto, EL
研究了4种单萜烯--樟脑、桉叶醇、柠檬烯和α-蒎烯对玉米根线粒体氧化代谢、种子萌发和初生根生长的影响。各单萜对呼吸作用的影响是不同的。浓度为0.05mM-1.0 mM的α-蒎烯，在没有外源添加基础呼吸的情况下，用由NADH、L-苹果酸、琥珀酸和L-谷氨酸组成的混合底物刺激呼吸。然而，当浓度高于1.0 mM时，α-蒎烯在没有ADP(基础呼吸)和存在ADP(耦合呼吸)的情况下都抑制呼吸。0.1 mM或以上的柠檬烯促进基础呼吸，平行抑制成对呼吸。Eucaliptol也有类似的促进作用，但在较高的浓度范围(1.0 mM或以上)。樟脑的活性较低。浓度为10 mM时，对基础呼吸有刺激作用，但对偶联呼吸无影响。在0.1~10.0 mM浓度范围内，柠檬烯对种子萌发和初生根生长无明显影响。樟脑和桉叶醇不抑制萌发，但降低5.0 mm及以上根的鲜重和干重。在10.0 mM浓度下，α-蒎烯对种子萌发和初生根鲜重均有抑制作用。结果表明，完整的种子和初级根对线粒体的敏感性低于分离的线粒体。在抑制种子萌发和/或初生根生长方面，相对亲油性较强的单萜烯α-蒎烯和柠檬烯的活性低于较易溶的含氧单萜烯樟脑和桉叶醇，尽管它们对分离线粒体的氧化代谢具有较高的活性。结果表明，单萜烯的溶解性可能是导致这些差异的主要因素。
The effects of four monoterpenes-camphor, eucaliptol, limonene, and alpha-pinene-on the oxidative metabolism of mitochondria isolated from maize root (Zea mays), on maize seed germination, and on primary root growth were investigated. The effects of individual monoterpenes on respiration were variable. alpha-Pinene concentrations of 0.05-1.0 mM stimulated respiration with a mixture of substrates composed of NADH, L-malate, succinate, and L-glutamate, and in the absence of exogenously added ADP (basal respiration). However, at concentrations higher than 1.0 mM, alpha-pinene inhibited respiration both in the absence (basal respiration) and presence of ADP (coupled respiration). Limonene at 0.1 mM or above stimulated basal respiration and inhibited in parallel the coupled respiration. Similar effects were promoted by eucaliptol, but at a higher concentration range (1.0 mM or above). Camphor was less active. At 10 mM concentration, it caused stimulation of basal respiration but did not affect coupled respiration. In the concentration range 0.1-10.0 mM, limonene was inactive on seed germination and primary root growth. Camphor and eucaliptol did not inhibit germination but reduced fresh and/or dry weight of roots at 5.0 mM and above. alpha-Pinene inhibited both seed germination and fresh weight of primary roots at 10.0 mM concentration. The results indicate that intact seeds and primary roots are less sensitive than isolated mitochondria. The relatively more lipophilic monoterpenes alpha-pinene and limonene had less activity than the more water-soluble oxygenated monoterpenes camphor and eucaliptol in inhibiting seed germination and/or primary root growth, despite the fact that they had a higher activity on the oxidative metabolism of isolated mitochondria. The Endings suggest that the solubility of monoterpenes may be the major factor implicated in these differences.