Differential Regulation of Ecdysteroidogenic P450 Gene Expression in the Silkworm, Bombyx mori
Differential Regulation of Ecdysteroidogenic P450 Gene Expression in the Silkworm, Bombyx mori
复制标题
DOI：
10.1271/bbb.70420
复制
发表时间：
2007-11
期刊：
影响因子：
--
通讯作者：
N. Yamanaka;Naoko Honda;Noe Osato;R. Niwa;A. Mizoguchi;H. Kataoka
中科院分区：
文献类型：
--
作者：
N. Yamanaka;Naoko Honda;Noe Osato;R. Niwa;A. Mizoguchi;H. Kataoka
研究了家蚕蜕皮激素合成P450基因表达的调控机制。Bommo-FMRFamide(BRFa)是一种前胸腺(PG)活动的神经抑制因子，可抑制促胸腺激素(PTTH)诱导的几个P450基因在前胸腺的表达。转录抑制剂抑制了PTTH诱导的P450基因的表达和BRFa的相反作用，而它们对蜕皮类固醇生成的短期影响保持不变。这一结果表明，这些因素对P450基因转录本的影响在较长的时间尺度上变得明显。此外，在长期的PG培养中观察到P450基因的自发表达，并被保幼激素抑制。这些结果很好地解释了PG中P450基因转录本的发育波动规律，表明在昆虫发育过程中，多种因素协同调节基础PG活性。
The regulatory mechanisms of ecdysteroidogenic P450 gene expression were investigated in the silkworm, Bombyx mori. Bommo-FMRFamide (BRFa), a neural suppressor of prothoracic gland (PG) activity, was found to suppress the expression of several P450 genes induced by prothoracicotropic hormone (PTTH) in the PG. A transcription inhibitor suppressed PTTH-induced expression of the P450 genes and the opposing effects of BRFa, while their short-term effects on ecdysteroidogenesis remained unchanged. This result suggests that the effects of these factors on the P450 gene transcripts become obvious on a longer time scale. Moreover, spontaneous expression of a P450 gene was observed in long-term PG culture, and was repressed by juvenile hormone. These results explain well the developmental fluctuation patterns of the P450 gene transcripts in the PG, indicating that multiple factors coordinate to regulate basal PG activity during insect development.