PHOTOCHEMICAL INTERNALIZATION OF BLEOMYCIN BEFORE EXTERNAL-BEAM RADIOTHERAPY IMPROVES LOCOREGIONAL CONTROL IN A HUMAN SARCOMA MODEL
PHOTOCHEMICAL INTERNALIZATION OF BLEOMYCIN BEFORE EXTERNAL-BEAM RADIOTHERAPY IMPROVES LOCOREGIONAL CONTROL IN A HUMAN SARCOMA MODEL
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DOI：
10.1016/j.ijrobp.2009.04.039
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发表时间：
2009-11-01
影响因子：
7
通讯作者：
Berg, Kristian
中科院分区：
文献类型：
--
作者：
Norum, Ole-Jacob;Bruland, Oyvind Sverre;Berg, Kristian
目的：本研究的目的是探索光化学内化和外束放疗联合应用对肿瘤生长的反应。光化学内化是一种技术，以提高利用治疗性大分子在癌症治疗中的光化学释放的内吞的大分子到cytosol.Methods和材料：人肉瘤异种移植TAX-I接种到裸鼠皮下。分别腹腔注射光敏剂AlPcS 2a和博来霉素48 h和30 min，然后以670 nm（20 J/cm（2））的半导体激光照射。光化学治疗后30分钟或7天，动物进行4戈伊的电离radiation.Results：使用博莱霉素作为辅助电离辐射的光化学内化增加的时间为17至33天的肿瘤进展相比，光动力学治疗结合电离辐射，以及与博莱霉素放化疗观察。观察到的副作用时，光化学内化的博莱霉素电离辐射前不久被消除分离的治疗models.Conclusion：博莱霉素结合电离辐射的光化学内化增加了时间的进展，并表现出最小的毒性，因此可能会减少总辐射剂量，同时避免长期的后遗症，从放疗获得局部肿瘤控制。(C)2009 Elsevier Inc.
Purpose: The aim of this study was to explore the tumor growth response of the combination photochemical internalization and external-beam radiotherapy. Photochemical internalization is a technology to improve the utilization of therapeutic macromolecules in cancer therapy by photochemical release of endocytosed macromolecules into the cytosol.Methods and Materials: A human sarcoma xenograft TAX-I was inoculated subcutaneously into nude mice. The photo-sensitizer AlPcS2a and bleomycin were intraperitoneally administrated 48 h and 30 min, respectively, before diode laser light exposure at 670 nm (20 J/cm(2)). Thirty minutes or 7 days after photochemical treatment, the animals were subjected to 4 Gy of ionizing radiation.Results: Using photochemical internalization of bleomycin as an adjunct to ionizing radiation increased the time to progression for the tumors from 17 to 33 days as compared with that observed with photodynamic therapy combined with ionizing radiation as well as for radiochemotherapy with bleomycin. The side effects observed when photochemical internalization of bleomycin was given shortly before ionizing radiation were eliminated by separating the treatment modalities in time.Conclusion: Photochemical internalization of bleomycin combined with ionizing radiation increased the time to progression and showed minimal toxicity and may therefore reduce the total radiation dose necessary to obtain local tumor control while avoiding long-term sequelae from radiotherapy. (C) 2009 Elsevier Inc.