Neurogenesis and Glial Proliferation Are Stimulated Following Diffuse Traumatic Brain Injury in Adult Rats
Neurogenesis and Glial Proliferation Are Stimulated Following Diffuse Traumatic Brain Injury in Adult Rats
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DOI：
10.1002/jnr.22635
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发表时间：
2011-07-01
影响因子：
4.2
通讯作者：
Morganti-Kossmann, M. Cristina
中科院分区：
文献类型：
--
作者：
Bye, Nicole;Carron, Sarah;Morganti-Kossmann, M. Cristina
虽然增加的神经发生已被描述在啮齿类动物模型的局灶性创伤性脑损伤（TBI），神经源性反应后发生的弥漫性TBI单纯局灶性损伤尚未检查到日期，尽管这种不同类型的脑损伤的普遍性TBI患者人群。在这里，我们描述了弥漫性TBI的创伤性轴索损伤（TAI）模型以及胶质细胞增殖反应后神经发生的多个阶段。在成年大鼠中使用冲击加速模型诱导TAI，并在创伤后1-4天或假手术中施用5-溴-20-脱氧尿苷（BrdU）以标记有丝分裂细胞。使用免疫组化BrdU结合表型特异性标记物，我们发现，增殖增加后TAI在侧脑室室下区和海马颗粒下区，虽然最终生产的新的齿状颗粒神经元在8周没有显着增强。此外，丰富的增殖和反应性星形胶质细胞，小胶质细胞，和多突胶质细胞检测到整个大脑后TAI，表明一个强大的神经胶质细胞反应发生在这个模型中，虽然非常少的新细胞在非神经原性脑区成为成熟的神经元。我们的结论是，弥漫性脑损伤刺激早期阶段的神经源性反应类似的模型描述的局灶性脑外伤。(C)2011 Wiley-Liss，Inc.
Although increased neurogenesis has been described in rodent models of focal traumatic brain injury (TBI), the neurogenic response occurring after diffuse TBI uncomplicated by focal injury has not been examined to date, despite the pervasiveness of this distinct type of brain injury in the TBI patient population. Here we characterize multiple stages of neurogenesis following a traumatic axonal injury (TAI) model of diffuse TBI as well as the proliferative response of glial cells. TAI was induced in adult rats using an impact-acceleration model, and 5-bromo-20-deoxyuridine (BrdU) was administered on days 1-4 posttrauma or sham operation to label mitotic cells. Using immunohistochemistry for BrdU combined with phenotype-specific markers, we found that proliferation was increased following TAI in the subventricular zone of the lateral ventricles and in the hippocampal subgranular zone, although the ultimate production of new dentate granule neurons at 8 weeks was not significantly enhanced. Also, abundant proliferating and reactive astrocytes, microglia, and polydendrocytes were detected throughout the brain following TAI, indicating that a robust glial response occurs in this model, although very few new cells in the nonneurogenic brain regions became mature neurons. We conclude that diffuse brain injury stimulates early stages of a neurogenic response similar to that described for models of focal TBI. (C) 2011 Wiley-Liss, Inc.