Storage iron kinetics. 3. Study of desferrioxamine action by selective radioiron labels of RE and parenchymal cells.
Storage iron kinetics. 3. Study of desferrioxamine action by selective radioiron labels of RE and parenchymal cells.
复制标题
储存铁动力学。
DOI：
10.5555/uri:pii:0022214373903090
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
D. Finch
中科院分区：
文献类型：
--
作者：
C. Hershko;J. Cook;D. Finch
摘要用多种选择性放射性铁探针研究去铁胺在大鼠体内的作用方式。使用了四个59铁标记的络合物，所有这些络合物都具有较高的初始肝脏摄取率。用放射自显影定量方法测定~(59)Fe在大鼠肝脏中的细胞分布，结果表明，其中两种络合物(受损的红细胞和沉淀的铁蛋白)可被RE细胞选择性地清除，而其余两种络合物(可溶性铁蛋白和结合珠蛋白的血红蛋白)标记肝实质细胞。DF可引起实质标记的排泄显著增加，而RE细胞探针排泄的增加并不比铁释放到转铁蛋白后再循环到实质中所能解释的多。从可溶性铁蛋白59Fe中螯合的放射性铁比例高于从血红蛋白59Fe中的放射性铁，这表明药物与实质铁蛋白储存相互作用，而不是与血红素分解代谢释放的铁相互作用。这进一步得到了DF诱导的排泄和不同实质放射性铁探针被同化到实质铁蛋白存储中的部分之间的密切关联的支持。
Abstract A variety of selective radioiron probes have been engaged to study the mode of action of desferrioxamine in the rat. Four 59 Fe-labeled complexes were used, all of which were characterized by a high initial hepatic uptake. A quantitative radioautographic method for determining the cellular distribution of 59 Fe in rat liver demonstrated that two of the complexes (damaged RBC and precipitated ferritin) were selectively removed by the RE cells, whereas the remaining two complexes (soluble ferritin and hemoglobin bound to haptoglobin) labeled the parenchymal cell of the liver. DF was found to induce a marked increase in the whole body excretion of parenchymal labels, whereas the increase in excretion of RE cell probes was no greater than could be accounted for by iron recirculation to the parenchyma after release to transferrin. A higher proportion of radioiron was chelated from soluble ferritin 59 Fe than from hemoglobin 59 Fe, suggesting an interaction of the drug with parenchymal ferritin stores and not with iron released by heme catabolism. This was further supported by the close correlation between DF-induced excretion and the fraction of the different parenchymal radioiron probes assimilated into parenchymal ferritin stores.