Decreased egg production in laying hens associated with infection with genotype 3 avian hepatitis E virus strain from China
Decreased egg production in laying hens associated with infection with genotype 3 avian hepatitis E virus strain from China
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产蛋鸡产蛋量下降与感染中国基因型 3 禽戊型肝炎病毒株有关
DOI：
10.1016/j.vetmic.2017.03.005
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发表时间：
2017-05-01
影响因子：
3.3
通讯作者：
Zhou, En-Min
中科院分区：
文献类型：
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作者：
Zhao, Qin;Liu, Baoyuan;Zhou, En-Min
为探讨产蛋量下降与禽戊型肝炎病毒（HEV）感染的关系，将30只健康的23周龄海兰褐蛋鸡随机分为3组，每组10只。接下来，使用来自中国的基因型3禽HEV毒株通过口鼻或静脉内途径使用500的50%鸡感染剂量来感染产蛋鸡。接种后14周（wpi）对所有母鸡进行尸检。粪便病毒脱落、病毒血症、血清转换、血清丙氨酸氨基转移酶（ALT）升高和肝脏病变表明，静脉（i.v.）经口、鼻接种，成功感染蛋鸡。与未接种组相比，静脉注射和口鼻接种组在接种后1周和2周表现出产蛋量下降，分别在接种后3周和8周达到产蛋量峰值。在两组中，12周内产量明显下降，总体下降幅度为10%至30%。此外，在7个产蛋量下降的蛋鸡场，已完成纽卡斯尔病、传染性支气管炎、禽流感H9 N2和H5 N1以及减蛋综合征病毒的疫苗接种方案。然而，使用检测禽HEV IgG抗体和RNA的试验在这些农场确认了禽HEV的循环。因此，实验和现场数据表明，禽戊型肝炎病毒感染单独作用可以解释所观察到的产蛋鸡产蛋量下降。(C)2017爱思唯尔B.V.保留所有权利。
To determine the relationship between decreased egg production and avian HEV infection, thirty healthy 23-week-old Hy-Line Variety Brown layer hens were randomly divided into 3 groups with 10 hens per group. Next, a genotype 3 avian HEV strain from China was used to inoculate laying hens via oronasal or intravenous routes using a 50% chicken infectious dose of 500. All hens were necropsied at 14 weeks postinoculation (wpi). Fecal virus shedding, viremia, seroconversion, serum alanine aminotransferase (ALT) increases and liver lesions showed that after intravenous (i.v.) and oronasal inoculation, the laying hens were successfully infected. Compared with the uninoculated group, the i.v. and oronasally inoculated groups exhibited egg production decreases at 1 wpi and 2 wpi, reaching peak production at 3 wpi and 8 wpi, respectively. In both groups, decreased production was evident for 12 weeks and overall decreases ranged from 10% to 30%. In addition, in the 7 field layer farms exhibiting decreased egg production, vaccination regimens had been completed against Newcastle disease, infectious bronchitis, avian influenza H9N2 and H5N1 and egg drop syndrome virus. However, circulating avian HEV was confirmed on these farms using tests to detect avian HEV IgG antibodies and RNA. Therefore, the experimental and field data indicate that avian HEV infection acting alone could account for observed decreases in egg production in laying hens. (C) 2017 Elsevier B.V. All rights reserved.