Detection of α-defensin in eosinophils in helminth-infected mouse model
Detection of α-defensin in eosinophils in helminth-infected mouse model
复制标题
蠕虫感染小鼠模型嗜酸性粒细胞中α-防御素的检测
DOI：
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发表时间：
2018
影响因子：
1.2
通讯作者：
M. Morimoto
中科院分区：
文献类型：
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作者：
A. Khatun;M. Sakurai;K. Okada;Y. Sakai;M. Morimoto
α-防御素（α-defensin）是由肠粘膜上皮细胞（如潘氏细胞）分泌的一种有效的抗菌肽，不仅影响细菌，而且影响寄生虫和真菌。最近，人类嗜酸性粒细胞也被证明可以产生α-防御素，但还没有在其他动物身上进行过研究。在这项研究中，我们试图检测α-防御素蛋白的嗜酸性粒细胞浸润肠道蠕虫感染的小鼠在Zamboni固定和免疫组织化学。蠕虫感染时肠粘膜浸润的嗜酸性粒细胞大多呈α-防御素阳性。α-防御素mRNA的表达水平是对照组的50倍。同时，Paneth细胞数量增加一倍，α-防御素荧光强度增强。这些结果表明，嗜酸性粒细胞也是α-防御素的重要生产者，如小鼠的Paneth细胞，嗜酸性粒细胞产生的α-防御素可能参与对抗蠕虫的防御机制。本研究所用的实验系统是研究嗜酸性粒细胞产生α-防御素的良好模型。
α-defensin is a potent antimicrobial peptide secreted from intestinal mucosal epithelial cells, such as Paneth cells, and affects not only bacteria but also parasites and fungi. Recently, human eosinophils have also been shown to produce α-defensin, but no studies have been done on other animals. In this study, we attempted to detect α-defensin protein in mouse eosinophils infiltrating the intestinal mucosa during a helminth infection using Zamboni fixation and immunohistochemistry. Most of the eosinophils infiltrating the intestinal mucosa during helminth infection were positive for α-defensin. The expression level of α-defensin mRNA was 50 fold that in the control. Meanwhile, the number of Paneth cells was doubled, and their α-defensin fluorescence intensity was increased. These results suggested that eosinophils are also important producers of α-defensin, such as Paneth cells in mice, and that α-defensin produced from eosinophils might be involved in defensive mechanisms against helminths. Moreover, the experimental system used in this study is a good model to study the generation of α-defensin by eosinophils.
DOI：
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发表时间：
2002
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Ayabe;D. Satchell;P. Pesendorfer;H. Tanabe;Carole L Wilson;S. Hagen;A. Ouellette
通讯作者：
T. Ayabe;D. Satchell;P. Pesendorfer;H. Tanabe;Carole L Wilson;S. Hagen;A. Ouellette
影响因子：
2.9
作者：
Jing, WG;Hunter, HN;Vogel, HJ
通讯作者：
Vogel, HJ