Strain-related cytokine profiles on the murine ocular surface in response to desiccating stress
Strain-related cytokine profiles on the murine ocular surface in response to desiccating stress
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DOI：
10.1097/ico.0b013e318033a729
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发表时间：
2007-06-01
期刊：
影响因子：
2.8
通讯作者：
Pflugfelder, Stephen C.
中科院分区：
文献类型：
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作者：
Corrales, Rosa M.;Villarreal, Arturo;Pflugfelder, Stephen C.
目的：观察干燥眼表应激对BALB/c和C57BL/6小鼠角膜上皮、结膜和泪液中炎性细胞因子水平的影响。方法：采用胆碱能阻断和干燥环境诱导BALB/c和C57BL/6小鼠实验性干眼（EDE）。实时聚合酶链反应测定细胞因子转录物水平。采用多重免疫珠法测定泪液中这些细胞因子的浓度。结果：实验干燥显著增加了C57BL/6小鼠角膜上皮和结膜中白细胞介素(IL)-1 α、IL-6和肿瘤坏死因子(TNF)- α转录本的表达。观察了菌株特异性泪液细胞因子谱的变化。与BALB/c小鼠相比，C57BL/6小鼠对干燥应激的反应中IL-1 α和tnf - α以及Th-1细胞因子IL-2、IL-12和干扰素- γ的泪液浓度显著高于BALB/c小鼠。Th-2细胞因子IL-4和IL-10在BALB/c撕裂中显著升高。结论：本研究表明，干燥应激以菌株依赖的方式增加角膜上皮和结膜中某些细胞因子的水平，C57BL/6小鼠泪液中Th-1细胞因子水平较高，而BALB/c小鼠泪液中Th-2细胞因子水平较高。
Purpose: To evaluate the effects of desiccating ocular surface stress on levels of inflammatory cytokines in the corneal epithelium, conjunctiva, and tear fluid of BALB/c and C57BL/6 mice.Methods: Experimental dry eye (EDE) was created in BALB/c and C57BL/6 mice by cholinergic blockade and exposure to a desiccating environment. Real-time polymerase chain reaction was performed to measure levels of cytokine transcripts. A multiplex immunobead assay was used to measure concentrations of these cytokines in tears.Results: Experimental dryness significantly increased the expression of interleukin (IL)-1 alpha, IL-6, and tumor necrosis factor (TNF)-alpha transcripts in the corneal epithelium and conjunctiva of C57BL/6 mice. Strain-specific changes in tear cytokine profiles were observed. C57BL/6 mice had significantly greater tear concentrations of IL-1 alpha and TNF-alpha and the Th-1 cytokines IL-2, IL-12, and interferon-gamma in response to desiccating stress than BALB/c mice. The Th-2 cytokines IL-4 and IL-10 were significantly greater in BALB/c tears.Conclusions: This study indicates that desiccating stress increases levels of certain cytokines in the corneal epithelium and conjunctiva in a strain-dependent fashion and that C57BL/6 mice had greater levels of Th-1 cytokines in their tears, whereas BALB/c mice had a greater increase in Th-2 cytokines.