Biogeographical distribution and regulation of methanotrophs in Chinese paddy soils
Biogeographical distribution and regulation of methanotrophs in Chinese paddy soils
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DOI：
10.1111/ejss.13200
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发表时间：
2021-11
影响因子：
4.2
通讯作者：
Wang‐ting Yang;Wei-qi Wang;Lidong Shen;Yanjing Bai;Xin Liu;Mao-hui Tian;Chun Wang;Yu-ling Yang-Yu
中科院分区：
文献类型：
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作者：
Wang‐ting Yang;Wei-qi Wang;Lidong Shen;Yanjing Bai;Xin Liu;Mao-hui Tian;Chun Wang;Yu-ling Yang-Yu
甲烷氧化菌对水稻生态系统的甲烷消耗有显着贡献。目前，中国水稻土中甲烷氧化菌的生物地理分布和调控仍知之甚少。在这里，我们调查了中国不同地理区域和三个气候带的水稻土中甲烷氧化菌的活性、丰度和群落结构，共20个采样点。结果表明，甲烷氧化活性和pmoA基因丰度分别为0.67~2.23 μmol CH4 g−1（干土）h−1和1.39×105~1.03×107拷贝g−1干土，甲烷氧化群落以甲基囊藻（II型）为主。在不同气候带中观察到异质分布的甲烷氧化菌群落，其特征是甲基球菌（I 型）相对丰度存在显着差异。此外，高和中等氮肥施用量表现出比低氮肥施用量显着更低的甲烷氧化活性。然而，在不同施氮水平之间没有观察到甲烷氧化丰度或群落组成的显着变化。此外，土壤铵含量、含水量、粘土含量、盐度、pH、年平均降水量和年平均温度被确定为影响甲烷氧化菌活性、丰度和群落组成的关键因素。总体而言，我们的结果显示了中国水稻土中甲烷氧化菌的生物地理分布，该分布在很大程度上受到气候条件、施氮量和土壤理化性质的影响。
Methanotrophs contribute significantly to methane consumption in paddy ecosystems. Currently, the biogeographical distribution and regulation of methanotrophs in Chinese paddy soils remain poorly understood. Here, we investigated the activity, abundance and community structure of methanotrophs in paddy soils from different geographical regions and three climate zones across China, with a total of 20 sampling sites. The results showed that the methanotrophic activity and pmoA gene abundance varied from 0.67 to 2.23 μmol CH4 g−1 (dry soil) h−1 and 1.39 × 105 to 1.03 × 107 copies g−1 dry soil, respectively, and the methanotrophic communities were dominated by Methylocystis (type II). Heterogeneously distributed methanotrophic communities were observed among different climate zones characterised by a significant variation of the relative abundance of Methylococcus (type I). Furthermore, high and intermediate rates of nitrogen (N) fertilisation showed a significantly lower methanotrophic activity than the low rates of N fertilisation. However, no significant variation of methanotrophic abundance or community composition was observed among different N fertilisation levels. In addition, the soil ammonium content, water content, clay content, salinity, pH, mean annual precipitation and mean annual temperature was identified as the key factors affecting the activity, abundance and community composition of methanotrophs. Overall, our results showed the biogeographical distribution of methanotrophs in Chinese paddy soils, which can be largely influenced by climate conditions, N fertilisation rates and soil physiochemical properties.