MAGI-1 interacts with β-catenin and is associated with cell-cell adhesion structures
MAGI-1 interacts with β-catenin and is associated with cell-cell adhesion structures
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DOI：
10.1006/bbrc.2000.2471
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发表时间：
2000-04-21
影响因子：
3.1
通讯作者：
James, GL
中科院分区：
文献类型：
--
作者：
Dobrosotskaya, IY;James, GL
膜相关鸟苷酸激酶（MAGUK）家族包括参与特化细胞-细胞连接形成的外周膜蛋白。MAGUK蛋白具有保守的结构域组成，包含PDZ、鸟苷酸激酶和SH 3或WW结构域。MAGI-1是最近鉴定的MAGUK蛋白家族的成员。迄今为止，已经表征了MAGI-1的三种剪接变体，包括MAGI-1a、-1b和-1c。MAGI-1b主要与粗膜部分相关。在这里，我们表明，第五PDZ域的MAGI-1b是必不可少的膜定位。我们还确定β-连环蛋白作为这个PDZ域的潜在配体。MAGI-1b在MDCK细胞中细胞-细胞连接形成期间与β-连环蛋白和E-钙粘蛋白形成复合物。与这一观察结果一致，MAGI-1b的GFP融合的显著部分定位于极化MDCK细胞的基底外侧膜。(C)北京大学出版社.
The family of membrane-associated guanylate kinases (MAGUK) comprises peripheral membrane proteins involved in the formation of specialized cell-cell junctions. MAGUK proteins possess a conserved domain composition, containing PDZ, guanylate kinase, and SH3 or WW domains. MAGI-1 is a recently identified member of the MAGUK protein family. Three splice variants of MAGI-1 have been characterized to date, including MAGI-1a, -1b, and -1c. MAGI-1b is predominantly associated with the crude membrane fraction. Here we show that the fifth PDZ domain of MAGI-1b is essential for membrane localization. We have also identified beta-catenin as a potential ligand for this PDZ domain. MAGI-1b forms complexes with beta-catenin and E-cadherin during the formation of cell-cell junctions in MDCK cells. In agreement with this observation, a significant portion of a GFP fusion of MAGI-1b localizes to the basolateral membrane of polarized MDCK cells. (C) 2000 Academic Press.