Thionin attached to a gold electrode modified with self-assembly of Mo6S9-XIX nanowires for amplified electrochemical detection of natural DNA
Thionin attached to a gold electrode modified with self-assembly of Mo6S9-XIX nanowires for amplified electrochemical detection of natural DNA
复制标题
DOI：
10.1016/j.bios.2010.01.035
复制
发表时间：
2011-01-15
影响因子：
12.6
通讯作者：
Li, Meixian
中科院分区：
文献类型：
--
作者：
Lin, Hong;Cheng, Huiming;Li, Meixian
我们展示了一种新型的电化学传感器，用于高灵敏度检测天然双链脱氧核糖核酸（dsDNA）的基础上，硫堇（Th）连接到Mo 6S 9-XIX纳米线（MoSI NWs）自组装在金电极上。传感检测是基于固定化Th的伏安响应的降低，由于Th与dsDNA通过嵌入结合。MoSI NW充当分子连接器以提供用于dsDNA的电化学检测的放大和导电感测平台，因为MoSI NW的末端和侧面处的许多硫原子允许与金形成共价键，并且具有负电荷的MoSI NW允许与Th静电结合。该传感器对dsDNA的检测限为0.62 ng/mL，比无MoSI纳米线时的检测限低3个数量级。操作简单，无标签。(C)2010 Elsevier B. V.保留所有权利。
We demonstrate a novel electrochemical sensor for highly sensitive detection of natural double-stranded deoxyribonucleic acid (dsDNA) based on thionin (Th) attached to Mo6S9-XIX nanowires (MoSI NWs) self-assembled on a gold electrode. The sensing detection is based on a decrease of the voltammetric response of the immobilized Th due to the binding of Th with dsDNA through intercalation. MoSI NWs act as molecular connectors to provide an amplification and conductive sensing platform for the electrochemical detection of dsDNA, because many sulfur atoms at the ends and sides of MoSI NWs permit covalent bonds to be formed with gold as well as MoSI NWs with negative charges allow electrostatic binding with Th. A determination limit of 0.62 ng/mL for dsDNA with this novel sensor is achieved, which is three orders of magnitude lower than that in the absence of MoSI NWs. The operation is simple and label-free. (C) 2010 Elsevier B.V. All rights reserved.