INTESTINAL ORGANOIDS FOR STUDYING THE EFFECTS OF LOW-DOSE/LOW-DOSE-RATE RADIATION
INTESTINAL ORGANOIDS FOR STUDYING THE EFFECTS OF LOW-DOSE/LOW-DOSE-RATE RADIATION
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DOI：
10.1093/rpd/ncac068
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发表时间：
2022-09-09
影响因子：
1
通讯作者：
Iwasaki, Toshiyasu
中科院分区：
文献类型：
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作者：
Fujimichi, Yuki;Otsuka, Kensuke;Iwasaki, Toshiyasu
肠干细胞的辐射反应因剂量和剂量率不同而不同；然而，其潜在机制尚不清楚。为了了解低剂量和低剂量率辐射的影响，作者建立了一个模拟体内环境和体外零星低剂量率辐射条件的类器官系统。1个辐照细胞的类器官形成电位和类器官中干细胞数量低于未辐照细胞；然而，差异并不显著，尽管1 gy辐照的干细胞在未辐照和辐照的干细胞混合类器官中表现出明显的生长劣势。此外，作者用x射线微束照射细胞并进行延时观察，发现照射后的细胞没有留在类器官中。这些结果表明，辐射诱导的干细胞竞争可以发生在肠道类器官中，并有助于低剂量率照射下的低癌症风险。
Radiation response differs depending on the dose and dose rate in intestinal stem cells; however, the underlying mechanisms are not clear. To understand the effects of low-dose and low-dose-rate radiation, the authors established an organoid system that mimics the in vivo environment and sporadic low-dose-rate irradiation conditions in vitro. Organoid-forming potential and the number of stem cells in the organoids derived from 1 Gy-irradiated cells were lower than those from non-irradiated cells; however, the difference was not significant, although 1 Gy-irradiated stem cells exhibited significant growth disadvantage in the mixed-organoid with non-irradiated and irradiated stem cells. Furthermore, the authors irradiated a cell with X-ray microbeams and performed time-lapse observations and found that irradiated cells did not remain in the organoid. These results suggest that radiation-induced stem cell competition can occur in intestinal organoids and contribute to a low risk of cancers at low-dose-rate exposures.