Magnolol suppresses the proliferation and invasion of cholangiocarcinoma cells via inhibiting the NF-κB signaling pathway
Magnolol suppresses the proliferation and invasion of cholangiocarcinoma cells via inhibiting the NF-κB signaling pathway
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DOI：
10.1016/j.biopha.2017.07.085
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发表时间：
2017-10-01
影响因子：
7.5
通讯作者：
Shen, Dong-Yan
中科院分区：
文献类型：
--
作者：
Zhang, Fu-Hui;Ren, Hong-Yue;Shen, Dong-Yan
Background: Magnolol has shown the potential anticancer properties against a variety of cancers. However, the role of magnolol in cholangiocarcinoma (CCA) cells is unknown. In this study, we assessed the effect of magnolol on the CCA cells.Methods: CCA cells were treated with magnolol in the absence or presence of TNF alpha, the activator for NF-kappa B. After co-incubation with magnolol, cell proliferation and growth were examined by MTT, colony formation and xenograft tumors; cell cycle was analyzed by flow cytometry; cell migration and invasion were detected by wound healing and transwell assays; the expression of PCNA, Ki67, CyclinD1, MMP-2, MMP-7 and MMP-9 and NF-kappa B pathway were evaluated by using Western blot.Results: Magnolol inhibited the abilities of CCA cell growth, migration and invasion accompanying with a decreased expression of PCNA, Ki67, MMP-2, MMP-7 and MMP-9 (all P < 0.05).Treatment: with magnolol induced cell cycle arrest in G1 phase with a downregulation of cell cycle protein CyclinD1 (all P < 0.05). In addition, magnolol suppressed the expression of p-I kappa B alpha and p-P65 and the effect of magnolol on CCA cells could be inhibited by TNF alpha.Conclusions: Magnolol could inhibit the growth, migration and invasion of CCA cells through regulation of NF-kappa B pathway, and these data indicate that magnolol is a potential candidate for treating of CCA. (C) 2017 Elsevier Masson SAS. All rights reserved.
