FIBRONECTIN AND POLYLYSINE REQUIREMENT FOR PROLIFERATION OF NEURO-BLASTOMA CELLS IN DEFINED MEDIUM
FIBRONECTIN AND POLYLYSINE REQUIREMENT FOR PROLIFERATION OF NEURO-BLASTOMA CELLS IN DEFINED MEDIUM
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DOI：
10.1016/0014-4827(80)90504-2
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发表时间：
1980-01-01
影响因子：
3.7
通讯作者：
SATO, GH
中科院分区：
文献类型：
--
作者：
BOTTENSTEIN, JE;SATO, GH
消除了B104 [大鼠]神经母细胞瘤细胞在限定培养基中增殖的血清预孵育要求。当胰蛋白酶或EGTA分离后将细胞直接接种到无血清的限定培养基中时，它们在粘附或保持附着于塑料基质上没有困难，但不能进行细胞分裂。将人血浆纤连蛋白添加到无血清限定培养基中，并在每次传代培养时用多聚赖氨酸预涂组织培养皿，以允许细胞分裂发生。仅在传代培养时需要纤连蛋白，无需在每次更换培养基时补充。如果细胞接种物遇到适当的基质，则可以在无血清确定成分培养基中进行克隆生长和连续传代培养。这些发现与纤维连接蛋白和带正电荷的基质在B104神经母细胞瘤细胞生长调节中的作用一致。这一完全确定的培养体系将对研究这些神经细胞的生长调节和分化非常有益。
The requirement of a serum preincubation for proliferation of B104 [rat] neuroblastoma cells in defined medium was eliminated. When cells were plated directly into serum-free defined medium after trypsin or EGTA detachment, they had no difficulty in adhering or remaining attached to the plastic substratum, but were incapable of cell division. The addition of human plasma fibronectin to serum-free defined medium and precoating the tissue culture dishes with polylysine at each subculture permitted cell division to occur. Fibronectin was only required at the time of subculture and did not need to be replenished at each medium change. Clonal growth and serial subculture are possible in serum-free defined medium, provided that the cell inoculum encounters the appropriate substratum. These findings are consistent with a role for fibronectin and a positively-charged substratum in the growth regulation of B104 neuroblastoma cells. This completely defined culture system will be of great benefit in studying the growth regulation and differentiation of these neuronal cells.